Abstract: Noninvasive microtest technique (NMT) is mainly controlled by a computer to measure the voltage at two points around the sample using selective microelectrodes. The mobile rates of ions or molecules can be obtained through a calibration curve, Nernst equation and Fick first diffusion law formula conversion based on the obtained voltage values. This technology shows some superiority such as keeping sample intact, higher distinguishability in time and space and determining several points at the same time, which can be used to realtime determine molecular or ion flow in organisms. This paper introduced the principle and characteristics of NMT used in environmental science. The applications of NMT in phytoremediation, plant resistance to soil salinization and biological monitoring in water were reviewed. Some shortages and perspectives of NMT in environmental science were also discussed.

Key words: subalpine meadow, species diversity, correlation analysis., pedigree diversity, &alpha