Abstract: To explore the response of gene expression of gill 14-3-3a, NKCCla, Apo-14 and Na⁺-K⁺-ATPaseβ in Mugil cephalus to salinity stress, polymerase chain reactionrestrained fragment length polymorphisms technique was applied. We found that NKCCla and Na⁺-K⁺-ATPaseβ protein synthesis was induced at the low salinity stress, with an obvious increase in expression level (P<0.01). However, the 14-3-3 gene mRNA expression level and Apo-14 gene mRNA expression level descended at the low salinity stress (P<0.05). The comprehensive evaluation index of multiple biomarkers was established based on the integrated biomarker responses (IBR). The IBR values of each gene indicated that the NKCCla and Na⁺-K⁺-ATPaseβ genes were suitable molecular biomarkers of the fish gill tissue for salinity stress assessment.

Key words: ecosystem services flow, quantitative, ecosystem service value, supply and demand coupling