Abstract: Microsatellite marker or simple sequence repeat (SSR) is an effective means in molecular genetics research, and the most popular molecular marker, because of its high polymorphism, large information contents, and conservative characteristics. However, SSR is a kind of special primer marker, and it is necessary to know a species DNA sequence to design the primers for PCR testing, a problem limiting microsatellite primer development. This paper reviewed the development strategies of representative microsatellite markers, such as genomic library screening, SSR enrichment, database search, relative species selection, TOMMI, and FIASCO, aimed to provide references to the microsatellite loci screening in molecular ecology research.

Key words: Elevated atmospheric CO₂, Rhizosphere microbes, Arbuscular mycorrhiza