Isolation of naphthalenedegrading bacteria and molecular detection of their naphthalene-catabolic genes

Abstract

Abstract: In this paper, 24 naphthalene-degrading bacterial isolates were isolated respectively from wastewater treatment facility’s activated sludge and from petroleum wastewater. The total DNA of these isolates were extracted, and hybridized with several naphthalene-catabolic genes. The results indicated that the bacterial isolates isolated from the two sources were quite different in their contained naphthalene-degrading genes. The isolates from petroleum wastewater contained naphthalene dioxygenase Fe-S protein large subunit genenahAc, salicylaldehyde dehydrogenase gene nahF and its duplicate genenahV, salicylate hydroxylase genenahG and its duplicate gene nahU, catechol 2,3-dioxygenase gene nahH, catechol 1,2-dioxygenase gene catA, and naphthalene chemotaxis protein gene nahY, while those from activated sludge only contained nahAc, nahF, nahG and catA, but nonahY,nahV, nahU andnahH.

Key words: Hibernating generation, Microvelia horvathi, Hunger tolerance, Temperature, Density

LI Yongjun, ZHAO Huabing, REN Heshan, LIANG Jing, CAI Baoli . Isolation of naphthalenedegrading bacteria and molecular detection of their naphthalene-catabolic genes[J]. cje.

[1]	LI Yinghao, LI Xin, REN Huimin, HUANG Chunyan, XUE Chunlei, LI Zhi, HAN Kang, JIAN Caiyuan, TIAN Lu, GUO Xiaoxia. Effects of biodegradable mulching film on soil water and heat conditions and water use efficiency of sugar beet. [J]. Chinese Journal of Ecology, 2026, 45(6): 1861-1869.
[2]	HE Tingting, MA Pengyu, CHEN Xiaomin, ZHAO Hanchen, YU Chao. The damages caused by high temperature stress to urban landscape plants and the prospects for countermeasures. [J]. Chinese Journal of Ecology, 2026, 45(4): 1143-1150.
[3]	ZHANG Wei, ZHENG Yunxiang, JI Lei, BO Shunqi, XIE Hanbin, WANG Junfu, LI Bicheng, YANG Gang. Longterm changes in species composition and population dynamics of amphibians in Shanghai. [J]. Chinese Journal of Ecology, 2026, 45(4): 1308-1314.
[4]	LIANG Yugang, LI Yujiao, KUANG Wei, ZHAO Yang, DAI Li, LUO Xianfu, FANG Baohua. Effects of crawfish powder application on soil bulk density and nutrients. [J]. Chinese Journal of Ecology, 2026, 45(3): 820-826.
[5]	LI Bo, WANG Dongrui, WANG Yong, LI Renmingxiu, XU Wei, PAN Qian, XU Liang, FENG Qiang. Activity rhythms of Reeve’s muntjac (Muntiacus reevesi) and its relationship with human activity in hilly and low mountains of Sichuan Province. [J]. Chinese Journal of Ecology, 2026, 45(2): 589-595.
[6]	ZE Handong, CHEN Yahui, CHEN Xuechu, YOU Wenhui, YANG Yue, ZHANG Zhao, YANG Hualei. Dynamics and modulators of soil organic carbon stocks in the northward-afforested mangroves in southern Zhejiang, China. [J]. Chinese Journal of Ecology, 2026, 45(1): 40-48.
[7]	HAO Zongran, SHEN Qinghua, ZHOU Weilong, WANG Dan, CHEN Yani, ZHANG Yulin, CHENG Xiangrong. Plant functional traits and their relationship with stand carbon density in Cunninghamia lanceolata forests with different sprout densities at the early stage of succession. [J]. Chinese Journal of Ecology, 2026, 45(1): 102-110.
[8]	ZHANG Jing, ZHANG Nan, ZHANG Huiyu, XIE Lili, ZHANG Dingyi. Response of root tiller development and yield formation of extremely-late sowing wheat to the interactions of planting density and nitrogen application. [J]. Chinese Journal of Ecology, 2026, 45(1): 192-201.
[9]	ZHOU Meng, LI Baozhan, CHEN Hui, CHAI Yuwei, YANG Yong, ZHANG Senyu, WANG Zeyi, CHANG Lei, HUANG Caixia. Effects of straw strip mulching coverage on winter wheat yield and soil water and heat distribution in Loess Plateau. [J]. Chinese Journal of Ecology, 2025, 44(8): 2618-2628.
[10]	WU Chijie, YU Lixiong, LIN Xiaoju, WANG Min, ZOU Yuanchao, XU Qiaoqing, DUAN Xinbin. The effect of temperature on swimming ability of four fish species. [J]. Chinese Journal of Ecology, 2025, 44(8): 2689-2695.
[11]	HU Shiyi, YANG Gairen, HUANG Zirong, YU Fang, DENG Wen, LI Lisha, DENG Yusong, LU Baopeng. The differences in carbon density between sunken and horizontal green space systems in Nakao River Wetland Park of Nanning City. [J]. Chinese Journal of Ecology, 2025, 44(8): 2717-2725.
[12]	YANG Liyan, SONG Meiling, WANG Yuqin, WANG Hongsheng, ZHOU Rui. Population characteristics of harmful organisms and their terrain influencing factors in the alpine meadow of Guoluo Prefecture, Qinghai Province. [J]. Chinese Journal of Ecology, 2025, 44(7): 2164-2173.
[13]	CHEN Siyi, LI Junjian, YIN Yaxin, SHAN Qiaobo, ZHENG Chunfang. Physiological mechanism of low temperature stress inhibiting photosynthesis in Aegiceras corniculatum seedlings. [J]. Chinese Journal of Ecology, 2025, 44(6): 1866-1873.
[14]	YANG Zhou, ZHANG Jianjun, ZHAO Jiongchang, HU Yawei, LI Yang, TANG Peng, WANG Bo. Effects of stand densities on growth characteristics of Pinus tabuliformis plantation in loess region of western Shanxi Province. [J]. Chinese Journal of Ecology, 2025, 44(5): 1427-1435.
[15]	YU Lei, LU Wen, SONG Peihong, YANG Qiu, YANG Huai, LIU Wenjie, LUAN Junwei, LIU Shirong. Carbon density and carbon sequestration potential of rubber plantations under different compound management patterns in Hainan Island. [J]. Chinese Journal of Ecology, 2025, 44(4): 1097-1103.

Isolation of naphthalenedegrading bacteria and molecular detection of their naphthalene-catabolic genes

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments