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生态学杂志 ›› 2024, Vol. 43 ›› Issue (7): 2158-2165.doi: 10.13292/j.1000-4890.202407.015

• 研究论文 • 上一篇    下一篇

园林废弃物生物炭对镉胁迫下油菜生理及光合荧光特性的影响

霍瑞朋1,张召娣1,徐雪芹1,王子铭1,刘曰华2,绳易坤1,宋香媛1,王子越1,王巨媛1*   

  1. 1聊城大学农学与农业工程学院, 山东聊城 252000; 2聊城大学地理与环境学院, 山东聊城 252000)

  • 出版日期:2024-08-10 发布日期:2024-07-08

Effects of garden waste biochar on physiological and photosynthetic fluorescence characteristics of rapeseed under cadmium stress.

HUO Ruipeng1, ZHANG Zhaodi1, XU Xueqin1, WANG Ziming1, LIU Yehua2, SHENG Yikun1, SONG Xiangyuan1, WANG Ziyue1, WANG Juyuan1*   

  1. (1Agricultural Science and Engineering School, Liaocheng University, Liaocheng 252000, Shandong, China; 2School of Geography and Environment, Liaocheng University, Liaocheng 252000, Shandong, China).

  • Online:2024-08-10 Published:2024-07-08

摘要: 为探究改性园林废弃物梧桐生物炭对镉污染土壤下油菜生理特性影响的作用机制,本研究采用盆栽试验,设置5个不同处理:CK、Cd、壳聚糖+Cd(K+Cd)、生物炭+Cd(C+Cd)、壳聚糖改性生物炭+Cd(Kc+Cd),测定油菜的生理、光合荧光特性。结果表明,壳聚糖、生物炭、壳聚糖改性生物炭的应用均对镉胁迫下油菜抗氧化系统(过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD))及渗透调节物质(丙二醛(MDA)、脯氨酸(Pro)、可溶性糖、可溶性蛋白)有积极作用。改性生物炭处理后对比镉处理油菜SOD活性下降28.1%;可溶性糖、可溶性蛋白含量分别增加187.7%、32.7%(P<0.05);有效提高叶绿素合成,光饱和点(LSP)、表观量子效率(AQY)、最大净光合速率(Pmax)和暗呼吸速率(Rd)等光合指标上升;最大荧光(Fm)、可变荧光(Fv)、PSⅡ最大光化学效率Fv/Fm、电子传递效率(ETR)、实际光化学效率产量(YⅡ)、光化学猝灭系数(qP)等荧光参数上升。壳聚糖、生物炭、改性生物炭均可减轻重金属诱导油菜的氧化应激,对镉胁迫下油菜光合特征、荧光特性有着促进作用,改性生物炭效果最好。


关键词: 园林废弃物生物炭, 油菜, 镉, 生理特性

Abstract: To explore the effects of chitosan-modified biochar originated from Platanus orientalis L. leaves on physiological characteristics of rapeseed under cadmium-contaminated soil, a pot experiment with five different treatments of CK, Cd, chitosan+Cd (K+Cd), biochar+Cd (C+Cd), chitosan-modified biochar+Cd (Kc+Cd) was conducted. The results showed that the applications of chitosan, biochar, chitosan-modified biochar all had positive impacts on antioxidant system (POD, CAT, SOD) and osmotic regulatory substances (malondialdehyde (MDA), proline (Pro), soluble sugar and soluble protein) in rapeseed subjected to cadmium stress. Under the Kc+Cd treatment, the SOD activity of rapeseed reduced by 28.1% compared with Cd treatment; the soluble sugar and soluble protein content significantly increased by 187.7% and 32.7%, respectively (P<0.05); the chlorophyll synthesis was effectively enhanced, light saturation point (LSP), light compensation point (LCP), apparent quantum efficiency (AQY), maximum net photosynthetic rate (Pmax) and dark respiration rate (Rd) were significantly increased. Maximum fluorescence (Fm), variable fluorescence (Fv), maximum photochemical efficiency of PSⅡ (Fv/Fm), electron transport efficiency (ETR), actual photochemical efficiency (YⅡ), and photochemical quenching coefficient (qP) were increased. Chitosan, biochar, and chitosan-modified biochar alleviated the oxidative stress of rape induced by heavy metals, promoted rape growth, and had positive effects on the photosynthetic characteristics and fluorescence characteristics of rape under cadmium stress, with the chitosan-modified biochar performing the best.


Key words: garden waste biochar, rape, cadmium, physiological characteristics