关键词: 氧化, 黑腹果蝇, H₂O₂, 卷翅, white基因

Abstract: Microorganisms play an important role in matter cycle and energy flow in aquaculture environment. Studies on microorganism diversity in aquaculture environment not only lay the foundation of disease control but also explore new microorganism resources. In this study, Illumina Miseq was used to analyze the structure characters of microbiome living in Dalian Takifugu rubripesculturing water indoors, water inlet point outdoors and bottom sludge respectively. For the three samples, 27164, 41146 and 30474 effective sequences were selected and utilized to perform bacterial diversity analysis. The results disclosed great bacterial diversities in the Takifugu rubripes aquaculture environment. Furthermore, the richness and diversity indices in the sediment samples were higher than that in the water samples. In total, 33 phyla were identified. In addition to Chloroflexi, Bacteroidetes, Planctomycetes, Cyanophyta, Acidobacteria, Proteobacteria, Fusobacterium, Verrucomicrobia, Firmicutes and Actinobacteria that have often been reported, other 23 phyla rarely reported were detected too. Although there were differences in microbial composition between these three samples, Proteobacteria and Bacteroidetes (68.33%-93.37%) were the dominant groups in all samples. This study reveals the bacterial diversity and abundance in Dalian Takifugu rubripes culturing environment, compares the bacterial community structures and provides guidance for healthy aquaculture of Takifugu rubripes.

Key words: H₂O₂, Drosophila melanogaster, curly wing, white gene, oxidation.