关键词: 垃圾填埋场覆盖土, Illumina MiSeq测序, 三氯乙烯(TCE), 微生物群落结构

Abstract: The rapid urbanization in China has caused a series of urban ecological problems, including surge in the number of motor vehicles, traffic congestion, worsening of traffic
pollutant emissions and energy consumption. Ecological footprint can be used as an effective indicator to measure the pressure of ecological environment brought by the development of urban transports. In this article, six megacities including Beijing, Shanghai, Tianjin, Hangzhou, Shenyang and Chengdu were chosen as the study objects, and the ecological footprints of urban transports in the six megacities were calculated from 2005 to 2012. The results showed that ecological footprints of urban transports in the six megacities were all increasing, and the pressure of urban transportation on ecological environment was also increasing every year. The indirect ecological footprint of fossil energy played a major role in the growing ecological footprint of urban traffic. The rapid growth of motor vehicles and cars and their high using times led to the continuous increase of energy consumption, which was the main reason for the growth of urban traffic ecological footprint. Improved development of green travel on rail transportation and slow traffic can effectively reduce the number of selfdriving travels in megacities, optimize the traffic travel structure, and ease the ecoenvironment pressure of urban traffic.

Key words: Illumina MiSeq sequencing, landfill cover soil, trichloroethylene, bacterial community structure.