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厚壳贻贝抗氧化酶活性和脂质过氧化物含量对Cd和Aroclor-1254胁迫的响应

彭玲1,曾江宁1,2**,黄伟1,陈全震1,刘晶晶1   

  1. (1国家海洋局海洋生态系统与生物地球化学重点实验室, 国家海洋局第二海洋研究所, 杭州 310012; 2浙江大学海洋学院, 杭州 310058)
  • 出版日期:2015-03-10 发布日期:2015-03-10

Effects of cadmium and Aroclor 1254 on antioxidant enzyme activities and lipid peroxidation levels in Mytilus coruscus.

PENG Ling1, ZENG Jiang-ning1,2**, HUANG Wei1, CHEN Quan-zhen1, LIU Jing-jing1   

  1. (1The Second Institute of Oceanography, SOA, Key Laboratory of Marine Ecosystem and Biogeochemistry of SOA, Hangzhou 310012, China; 2 Ocean College, Zhejiang University, Hangzhou 310058, China)
  • Online:2015-03-10 Published:2015-03-10

摘要:

以厚壳贻贝(Mytilus coruscus)为目标生物,研究了不同浓度Cd2+(0.194、0.388、0.775 mg·L-1)、Aroclor 1254 (0.005、0.01、0.05 mg·L-1)单一和复合胁迫对其消化盲囊抗氧化酶活性及脂质过氧化产物丙二醛(MDA)含量的影响。结果表明:(1)单一和复合胁迫均可导致厚壳贻贝抗氧化酶的活性先升高后抑制。高浓度组(0.775 mg·L-1 Cd2+、0.05 mg·L-1 Aroclor 1254)在单一胁迫第1天时,抗氧化酶的活性即达峰值。抗氧化酶活性的下降幅度和速率与胁迫物质作用时间和浓度呈正相关。(2)SOD、GSH-Px对胁迫的敏感性高于CAT。(3)各胁迫处理组MDA含量随胁迫时间延长均显著升高(P<0.01)。单一胁迫的效应与剂量和时间呈正相关。(4)复合胁迫的效应强于单一胁迫。推测高于环境水平的Cd2+、Aroclor 1254对厚壳贻贝可产生明显氧化胁迫,抗氧化防御系统可以作为海洋环境重金属有机物复合污染监测的潜在生物标志物。
 

关键词: 大兴安岭, 气候变化, 人为火, 北方森林, 空间点格局分析

Abstract: The effects of antioxidant enzymes on the digestive diverticula of Mytilus coruscus were studied at different concentrations of cadmium (0.194, 0.388 and 0.775 mg·L-1) and Aroclor 1254 (0.005, 0.01 and 0.05 mg·L-1) under single and combined stress conditions. The results are as follows: (1) Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) activities changed similarly, being first increased and then inhibited under single and combined stress conditions. The highest dose groups (0.775 mg·L-1 of Cd2+, 0.05 mg·L-1 of Aroclor 1254) reached the peak values of antioxidant defense systems earlier on the first day of single stress. The descending range and rate of antioxidant enzymes activities were positively correlated with the stress time and concentration of stress material. (2) The stress sensitivities of SOD and GSH-Px were higher than that of CAT. (3) The content of malonaldehyde (MDA) of each dose group was significantly increased (P<0.01) as the stress time was prolonged. A positive correlation was found between effect of single stress and stress dose or time. (4) The effect of combined stress was stronger than that of single stress. It could be inferred that oxidative stress in M. coruscus would be produced by exposure to cadmium and polychlorinated biphenyl above ambient levels. The antioxidant defense systems could act as biomarkers to monitor marine multiple contamination of heavy metals and organics.

Key words: boreal forest, human-caused fire, spatial point pattern analysis, climate change, Great Xing’an Mountains