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不同烟粉虱抗性辣椒对外源茉莉酸甲酯的生理响应

吴晓霞1,何菁3,周福才2,3*,陈学好3,杨爱民3,张海波3   

  1. 1扬州大学生物科学与技术学院, 江苏扬州 225009;2农业部农产品质量安全生物性危害因子(动物源)控制重点实验室, 江苏扬州 225009;3扬州大学园艺与植物保护学院, 江苏扬州 225009)
  • 出版日期:2019-03-10 发布日期:2019-03-10

Physiological responses of different whitefly resistant peppers to exogenous methyl jasmonate.

WU Xiao-xia1, HE Jing3, ZHOU Fu-cai2,3*, CHEN Xue-hao3, YANG Ai-min3, ZHANG Hai-bo3   

  1. (1Collage of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China; 2Quality and Safety of Agricultural Products Biological Hazard Factor (Animal Source) Control Key Laboratory, Ministry of Agriculture, Yangzhou 225009, Jiangsu, China; 3College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu, China).
  • Online:2019-03-10 Published:2019-03-10

摘要: 以两种不同抗性辣椒品种“苏椒13号”(感烟粉虱)和“新一代三鹰椒”(抗烟粉虱)为对象,研究外源茉莉酸甲酯处理对辣椒叶片叶绿素含量、可溶性糖含量、可溶性蛋白含量、酚类化合物含量以及过氧化物酶和多酚氧化酶活性等的影响。结果表明:不同抗性品种辣椒叶片叶绿素含量、可溶性糖含量、酚类化合物含量以及过氧化物酶和多酚氧化酶的活性均随外源茉莉酸甲酯处理浓度的升高而呈现先升后降的趋势,茉莉酸甲酯响应峰值浓度在10~100 μmol·L-1,1000 μmol·L-1处理组的各生理指标值均显著低于对照;适宜浓度处理条件下抗虫品种“新一代三鹰椒”的可溶性糖含量、酚类化合物含量显著高于感虫品种“苏椒13号”,但可溶性蛋白在处理浓度范围内无显著性变化;受外源茉莉酸甲酯诱导,“苏椒13号”的过氧化物酶及多酚氧化酶的活性均显著高于“新一代三鹰椒”,可溶性蛋白含量呈现下降趋势,但在处理浓度范围内无显著性差异;适宜浓度的外源茉莉酸甲酯可诱导辣椒体内的抗虫相关物质向着有利于提高抗烟粉虱的方向改变,不同抗性辣椒品种对外源茉莉酸甲酯的响应程度不同。

关键词: NO, NO2, 地气交换, CO2浓度增高, 静态暗箱法

Abstract: In this study, we examined the physiological responses of two pepper varieties with different whitefly resistance, Sujiao 13 (susceptible variety) and Xinyidai Shanyingjiao (resistant variety), to the exogenous methyl jasmonate treatment. We measured the dynamic changes of chlorophyll content, soluble sugar content, soluble protein content, phenolic compound content, and peroxidase and polyphenol oxidase activity. Our results showed that chlorophyll content, soluble sugar content, phenolic compound content, and the activities of peroxidase and polyphenol oxidase in leaves of two cultivars all exhibited a trend of first increase and then decrease with the increasing concentration of methyl jasmonate. The peak response concentrations were among 10-100 μmol·L-1. All physiological indices in 1000 μmol·L-1 treated groups were significantly lower than those in the control. Under the treatment of appropriate concentration of methyl jasmonate, the contents of soluble sugar and phenolic compounds in the resistant variety were significantly higher than those in the susceptible variety. The soluble protein content showed no significant difference in treated groups of different concentrations of methyl jasmonate. The peroxidase and the polyphenol oxidase activities in the susceptible variety were substantially stimulated by exogenous methyl jasmonate, and were higher than that in the resistant variety. The soluble protein content showed a downward trend, but with no significant difference among different treatments. Our results indicate that an appropriate concentration of exogenous methyl jasmonate can induce the adjustment of insect-resistant substances in peppers and improve the insect resistance of pepper. The responses of different resistant varieties to the exogenous methyl jasmonate are different.