关键词: 有机胶结物质, 土壤团聚体, 理论模型

Abstract: In this paper, three key factors of cryopreservation by vitrification, i.e., cryoprotective solution application, dehydration time of vitrification solution, and rapid freezing, were installed to study the effects of cryopreservation by vitrification on the viability of Zostera marina seeds. Cryoprotective solution application and dehydration time had no significant effects on the viability of the seeds. When exposed to rapid freezing, the viability of the seeds was significantly lower than that of the control, while cryoprotective solution application and dehydration time showed no significant protective effects on the viability of the seeds. Without cryoprotective solution application, the viability of the seeds subjected to 4 h dehydration was significantly lower than that of the seeds subjected to other lengths of dehydration time. With cryoprotective solution application, no significant difference was found between the viability of the seeds subjected to 4 h dehydration and that of the seeds subjected to other lengths of dehydration time. These findings indicated the necessity of cryoprotective solution application to seeds prior to the long period dehydration by vitrification. This study provides a reference for the cryopreservation of Z. marina seeds.