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生态学杂志 ›› 2010, Vol. 29 ›› Issue (11): 2168-2174.

• 研究报告 • 上一篇    下一篇

四川六个厚朴种群遗传结构

于华会1,杨志玲1**,刘若楠1,林杰君2   

  1. 1中国林业科学研究院亚热带林业研究所, 浙江富阳 311400|2浙江师范大学生态研究所, 浙江金华 321004
  • 出版日期:2010-11-08 发布日期:2010-11-08

Genetic structure of six Magnolia officinalis populations in Sichuan Province.

YU Hua-hui1, YANG Zhi-ling1, LIU Ruo-nan1, LIN Jie-jun2   

  1. 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China|2Institute of Ecology, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
  • Online:2010-11-08 Published:2010-11-08

摘要: 利用ISSR技术对四川6个野生厚朴种群的遗传结构进行了分析,以评估野生厚朴资源的遗传现状。10条ISSR引物共测到114个位点,其中多态位点93个,多态位点百分率(PPB)为81.58%,Nei遗传多样性指数(H)和Shannon信息指数(I)分别为0.320和0.469,表明厚朴物种水平具有较高的遗传多样性;相比之下,种群水平遗传多样性则相对较低(PPB、H、I分别平均为48.25%、0.189和0.277)。分子变异分析(AMOVA)表明,种群内部和种群间均存在极显著遗传分化(P<0.001),其中,36.82%的变异存在于种群间,63.18%存在于种群内,种群间的基因分化系数(GST)为40.42%。厚朴种群间的基因流(Nm)为0.737,平均遗传距离为0.211,算术加权平均数法(UPGMA)显示厚朴6个种群被分为3大类群。Structure分析表明,厚朴种群间遗传结构具有独立性特点。

关键词: 树木年轮, 大气, δ13C, 模型

Abstract: In this paper, inter-simple sequence repeats (ISSR) technique was applied to study the genetic diversity and genetic differentiation of six Magnolia officinalis populations in Sichuan Province. For the 159 individuals of the six populations, 10 ISSR primers yielded a total of 114 bands sized 150-2000 bp, among which, 93 bands (81.58%) were polymorphic. The number of the bands per primer yielded varied from 10 to 13, with an average of 11.4 bands. A relatively low genetic diversity was detected at population level (the percentage of polymorphic loci PPB=48.25%, Nei’s genetic diversity H=0.189, and Shannon’s information index  I=0.277). However, the genetic diversity at species level was relatively high (PPB=81.58%, H=0.320, and I=0.469). The coefficient of genetic differentiation (GST) among populations was 40.42%, and the results of molecular variance analysis indicated that the genetic differentiation occurred mainly within populations (63.18%). The gene flow between M. officinalis populations was 0.737, and the average genetic distance was 0.211. The six populations were divided into three groups, according to UPGMA. Structure analysis showed that the genetic structure of the M. officinalis populations had the characteristics of independence.

Key words: Tree ring, Atmosphere, 13C, Model