Tpb55菌株对烟草赤星病菌的影响及其在烟草叶表的定殖

生态学杂志

Tpb55菌株对烟草赤星病菌的影响及其在烟草叶表的定殖

张成省¹;孔凡玉¹;刘朝科²;杜卫民³;李文刚³;王静¹

¹中国农业科学院烟草研究所，山东青岛 266101;²川渝中烟工业公司，成都 610017;³四川省烟草专卖局，成都 610017

收稿日期:2008-11-07 修回日期:1900-01-01 出版日期:2009-06-10 发布日期:2009-06-10

Inhibitory effects of Bacillus subtilis Tpb55 strain on Alternaria alternata growth and the colonization of the strain on tobacco leaf.

ZHANG Cheng-sheng¹;KONG Fan-yu¹;LIU Chao-ke²;DU Wei-min³;LI Wen-gang³;WANG Jing¹

¹Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China;²China Tobacco Chuanyu Industrial Corporation, Chengdu610017, China;³Sichuan Tobacco Monopoly Administration, Chengdu 610017,China

Received:2008-11-07 Revised:1900-01-01 Online:2009-06-10 Published:2009-06-10

摘要/Abstract

摘要： 采用生长速率法及分生孢子萌发法测定了烟草叶围细菌Tpb55菌株对烟草赤星病菌的影响。结果表明：Tpb55能分泌抗菌物质，抑制烟草赤星病菌的菌丝生长和分生孢子萌发，破坏菌丝和分生孢子形态，使菌丝和分生孢子出现细胞原生质收缩、肿大呈泡囊状、细胞壁破损导致原生质外泄等。温室和田间定殖作用测定结果表明：0～30 d内，Tpb55菌株在烟草叶表定殖菌量分别为23×10⁶～189×10⁶ cfu·g^-1鲜质量和1.87×10⁶～179.33×10⁶ cfu·g^-1鲜质量，其中，接种后3～8 d，Tpb55定殖数量较高且基本稳定，随后定殖数量逐渐下降。

关键词: 黄足黄守瓜, 黄足黑守瓜, 瓜类作物, 取食行为, 葫芦素

Abstract: By the methods of determining mycelial growth rate and conidial spore germination, the effects of Bacillus subtilis Tpb55 strain on Alternaria a lternata growth were studied, and the colonization of the strain on tobacco le af was examined under greenhouse and field conditions. It was shown that the ant ifungal substance secreted by strain Tpb55 inhibited the mycelial growth and the conidial spore germination of A. alternata, and damaged the pathogen’s mor phology. The protoplasm of hyphal and conidia cells shrunk, the cells swelled, a nd the cell wall damaged, inducing the leak-out of protoplasm. Within the 0〖KG-*2〗-〖KG-*7〗30 days of inoculation, the colonization rate of strain Tpb5 5 on tobacco leaf was 23×106〖KG-*2〗-〖KG-*7〗189×106 cfu·g-1 fresh mass in greenhouse and 187×106〖KG-*2〗-〖KG-*7〗17933×106 cfu·g-1 fresh mass in field, being maintained a t a high and stable level in the first 3〖KG-*2〗-〖KG-*7〗8 days of inoculation and decreased rapidly thereafter.

Key words: Aulacophora fermoralis chinensis, Aulacophora cattigarensis, Melon crop, Feeding behavior, Cucurbitacin

张成省;孔凡玉;刘朝科;杜卫民;李文刚;王静. Tpb55菌株对烟草赤星病菌的影响及其在烟草叶表的定殖[J]. 生态学杂志.

ZHANG Cheng-sheng¹;KONG Fan-yu¹;LIU Chao-ke²;DU Wei-min³;LI Wen-gang³;WANG Jing¹. Inhibitory effects of Bacillus subtilis Tpb55 strain on Alternaria alternata growth and the colonization of the strain on tobacco leaf.[J]. cje.

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