松花湖铜绿微囊藻无菌株和单藻株生长因素的研究

生态学杂志 ›› 2005, Vol. ›› Issue (5): 518-522.

松花湖铜绿微囊藻无菌株和单藻株生长因素的研究

王霞^1,2,3, 吕宪国¹, 张学林¹, 张竹青²

1. 中国科学院东北地理与农业生态研究所, 长春, 130012;
2. 吉林省环境监测中心站, 长春, 130011;
3. 中国科学院研究生院, 北京, 100039

收稿日期:2004-03-31 修回日期:2004-06-15 出版日期:2005-05-10
通讯作者: 王霞,女,1970年生,博士生.主要从事水生态学和环境生物学方面的研究工作.E-mail:wangx16@mail.neigae.ac.cn.
基金资助:
中国科学院东北地理与农业生态研究所学科前沿领域资助项目(KZCX3-SW-NA-01)

Growth factors of unialgal strain and axenic strain of Microcystis aeruginosa in Songhua Lake

WANG Xia^1,2,3, LU Xianguo¹, ZHANG Xuelin¹, ZHANG Zhuqing ²

1. Northeast Institute of Geography and Agriculture Ecology, Chinese Academy of Sciences, Changchun 130012, China;
2. Environment Monitoring Center of Jilin Province, Changchun 130011, China;
3. Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Received:2004-03-31 Revised:2004-06-15 Online:2005-05-10

摘要/Abstract

摘要： 用毛细滴管洗净法从松花湖分离纯化出铜绿微囊藻(Microcystis aeruginosa)的单藻株和无菌株,研究了温度、照度、N、P、Fe各种因素对单藻株和无菌株生长的影响。结果表明,各种因素对单藻株和无菌株最大比增长率的影响差别不大,单藻株和无菌株分别在温度33℃,照度5000Lx(2000Lx)、NO₃^--N浓度1.2mg·L^-1,PO₄^3--P浓度0.06mg·L^-1,柠檬酸铁铁浓度0.05mg·L^-1(0.04mg·L^-1)时,达到最大比增长率。在一定浓度范围内,随着N、P、Fe浓度的增加,单藻株比无菌株的最大增长量高得多,可见附着细菌的存在,能促进铜绿微囊藻的生长。从松花湖湖水中N、P和Fe含量以及温度和照度的情况看,松花湖的某些区域已经基本具备了水华发生的条件。

关键词: 秸秆还田, 土壤呼吸, 土壤微生物量

Abstract: Employing capillary-pipette washing method,the unialgal strain and axenic strain of Microcystis aeruginosa from Songhua Lake were isolated and purified,and effects of temperature,illuminance,nitrogen,phosphorus,and iron on their growth were investigated.The results showed that there was no significant difference under the influences of temperature,illuminance,nitrogen,phosphorus,and iron on the maximum specific growth rate of the unialgal strain and axenic strain.It reaches the maxirnum specific growth rate under the conditions of 33 ℃ of temperature,5 000 Lx (2 000 Lx) of illuminance,1.2 mg·L^-1 of NO₃^--N,0.06 mg·L^-1 of PO₄^3--P and 0.05 mg·L^-1 (0.04 mg·L^-1) of ferric citrate.With the increase of N,P and Fe concentrations,maximum growth of the unialgal strain was much higher than that of the axenic strain.Thus,it would be able to improve the growth of M.aeruginosa if the associated bacteria presented.In view of N,P and Fe concentrations and the conditions of temperature and illumination in Songhua Lake,there exist the conditions of water-bloom in somepart of Songhua Lake.

Key words: Crop residue, Soil respiration, Soil microbial biomass

中图分类号:

Q178.51

王霞, 吕宪国, 张学林, 张竹青. 松花湖铜绿微囊藻无菌株和单藻株生长因素的研究[J]. 生态学杂志, 2005, (5): 518-522.

WANG Xia, LU Xianguo, ZHANG Xuelin, ZHANG Zhuqing . Growth factors of unialgal strain and axenic strain of Microcystis aeruginosa in Songhua Lake[J]. cje, 2005, (5): 518-522.

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