杉木人工林土壤真菌反硝化功能基因丰度特征及其环境影响因子

doi:10.13292/j.1000-4890.202602.030

生态学杂志 ›› 2026, Vol. 45 ›› Issue (2): 538-543.doi: 10.13292/j.1000-4890.202602.030

杉木人工林土壤真菌反硝化功能基因丰度特征及其环境影响因子

陈琦润¹,林欣宏¹,邓米林²,杨浩¹,张晗烁¹,郑勇¹,林永新^1*

（¹福建师范大学地理科学学院，福州 350117； ²中国科学院华南植物园，广州 510650）

出版日期:2026-02-10 发布日期:2026-08-01

Characteristics and environmental driving factors of fungal denitrification functional gene abundance in soils of Chinese fir plantation in Fujian Province.

CHEN Qirun¹, LIN Xinhong¹, DENG Milin², YANG Hao¹, ZHANG Hanshuo¹, ZHENG Yong¹, LIN Yongxin^1*

(¹School of Geographical Sciences, Fujian Normal University, Fuzhou 350117, China; ²South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China).

Online:2026-02-10 Published:2026-08-01

摘要/Abstract

摘要： 亚热带森林土壤是真菌反硝化发生的热点区域，而真菌反硝化过程是土壤氧化亚氮（N₂O）产生的重要途径。然而，亚热带杉木人工林反硝化真菌功能基因（FnirK）的分布特征及其环境驱动因子尚不清楚。本研究采集福建省9个地点杉木人工林土壤共108个样品，利用实时荧光定量PCR测定FnirK基因丰度。结果表明：杉木人工林土壤FnirK基因丰度呈现较大的空间异质性。三明市沙县官庄国有林场土壤FnirK基因丰度最高，为19.75×10⁸ copies·g^-1，而龙岩市漳平五一国有林场土壤FnirK基因丰度最低，为3.94×10⁸ copies·g^-1，9个林场FnirK基因丰度的平均值为11.9×10⁸ copies·g^-1。随机森林分析表明，土壤pH值是影响FnirK基因丰度最重要的环境因子，解释度为21.02%。相关性分析表明，FnirK基因丰度与土壤pH值及年平均降水量（MAP）呈显著正相关，与土壤可溶性有机碳（DOC）和总碳（TC）呈显著负相关。综上，福建省杉木人工林土壤反硝化真菌功能基因FnirK丰度存在较大空间异质性，土壤pH值、DOC含量和MAP是影响其分布的主要环境因子。

关键词:

真菌反硝化, FnirK基因, 亚热带森林, 氧化亚氮

Abstract: Subtropical forest soil is a hot spot for fungal denitrification, which is an important pathway for the production of nitrous oxide (N₂O) in soils. However, the distribution pattern of the denitrifying fungal functional gene (FnirK) and its environmental drivers in subtropical Chinese fir plantations remain unclear. In this study, a total of 108 soil samples were collected from nine Chinese fir plantations in Fujian Province, and the abundance of FnirK gene was measured using real0time quantitative PCR. The results showed that the physicochemical properties and the abundance of FnirK gene exhibited significant spatial heterogeneity. The FnirK gene abundance in Guanzhuang State-owned Forest Farm, Shaxian County, Sanming City was the highest, at 19.75×10⁸ copies·g^-1, while its abundance in Wuyi State-owned Forest Farm, Zhangping City, Longyan City was the lowest, at 3.94×10⁸ copies·g^-1. The average abundance of FnirK gene in the nine plantations was 11.9×10₈ copies·g^-1. Random forest analysis indicated that soil pH was the most important environmental factor affecting FnirK gene abundance, explaining 21.02% of the total variations. FnirK gene abundance was significantly positively correlated with soil pH and mean annual precipitation (MAP), and significantly negatively correlated with soil dissolved organic carbon (DOC) and total carbon (TC). In conclusion, there is significant spatial heterogeneity in the abundance of denitrifying fungal functional gene FnirK in the soils of Chinese fir plantations in Fujian Province, with soil pH, DOC content, and MAP being the main factors influencing its distribution.

Key words: fungal denitrification, FnirK gene, subtropical forest, nitrous oxide

陈琦润, 林欣宏, 邓米林, 杨浩, 张晗烁, 郑勇, 林永新. 杉木人工林土壤真菌反硝化功能基因丰度特征及其环境影响因子[J]. 生态学杂志, 2026, 45(2): 538-543.

CHEN Qirun, LIN Xinhong, DENG Milin, YANG Hao, ZHANG Hanshuo, ZHENG Yong, LIN Yongxin. Characteristics and environmental driving factors of fungal denitrification functional gene abundance in soils of Chinese fir plantation in Fujian Province.[J]. Chinese Journal of Ecology, 2026, 45(2): 538-543.

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杉木人工林土壤真菌反硝化功能基因丰度特征及其环境影响因子

Characteristics and environmental driving factors of fungal denitrification functional gene abundance in soils of Chinese fir plantation in Fujian Province.

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