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小花草玉梅种子萌发过程中常见内源激素含量的变化及萌发机制

葛文静,卜海燕*,王学经,夏玉斌,杜国祯   

  1. (兰州大学生命科学学院, 草地与农业生态系统国家重点实验室, 兰州 730000)
  • 发布日期:2020-06-10

Changes of endogenous hormone contents during seed germination of Anemone rivularis var. floreminore and germination mechanisms.

GE Wen-jing, BU Hai-yan*, WANG Xue-jing, XIA Yu-bin, DU Guo-zhen   

  1. (State Key Laboratory of Grassland AgroEcosystems, School of Life Sciences, Lanzhou University, Lanzhou 730000, China).
  • Published:2020-06-10

摘要: 植物内源激素在调节种子休眠和萌发过程中具有极其重要的作用。本研究运用反式高效液相色谱(RP-HPLC)与紫外检测器联用的方法对小花草玉梅干种子、吸胀种子和露白种子中的赤霉素GAs、脱落酸ABA、玉米素ZT和生长素IAA含量进行了检测,旨在研究植物内源激素水平在种子萌发过程中的变化是否直接关系着小花草玉梅种子在光照或黑暗条件下的萌发能力。结果显示,种子吸胀过程中,光照促进了ZT同时抑制了ABA的积累,并且ZT对ABA萌发抑制作用的解除也受光的促进,露白种子中的ABA/ZT,ABA/GAs和(ABA+IAA)/(GAs+ZT)水平在黑暗条件下高于光照条件,上述均是导致光照条件下种子萌发率较高的重要原因;相对于干种子,IAA含量在种子吸胀初期急速下降,(ABA+IAA)/(GAs+ZT)在种子萌发过程中有所降低,而ABA/GAs却表现出明显的上升趋势;各激素水平所受光照的影响均在种子开始露白时显著减弱,另外,吸胀第9天是小花草玉梅种子萌发过程中激素变化的一个关键的时间节点。总之,种子萌发并非直接关系着GA含量的升高和ABA含量的降低。因光照直接促进了小花草玉梅种子的萌发,本研究认为高寒草甸充足的光照和较强的太阳辐射通过调节种子内源激素水平在一定程度上对种子萌发过程中的休眠解除具有重要作用。

关键词: Ross-Li模型, 聚集度系数\ MODIS BRDF

Abstract: Seed germination and dormancy are regulated by endogenous hormones. In this study, we investigated whether endogenous hormone levels are associated with seed germination ofAnemone rivularisvar.floreminore in 12 h/12 h light/dark and 24 h dark. The endogenous hormone contents of abscisic acid (ABA), gibberellins (GAs), transzeatin (ZT), and indole-3-acetic acid (IAA) were detected in dry, imbibed and germinated (emerged radicle) seeds using reversed phase highperformance liquid chromatography (RP-HPLC) with ultraviolet detection. Our results showed that light stimulated ZT and suppressed ABA accumulation before seedling emergence, and promoted the release of ZT to ABA inhibition during seed germination. The ratios of ABA/ZT, ABA/GAs and (ABA+IAA)/(GAs+ZT) in germinated seeds were higher in the dark than in the light. These results were consistent with the fact that A. rivularis var. floreminore seeds germinated more efficiently in the light than in the dark. Compared to that in dry seeds, IAA content rapidly declined in early imbibition in both light and dark periods, (ABA+IAA)/(GAs+ZT) ratio decreased significantly and ABA/GAs ratio increased. The effects of light on hormone levels were weakened when the first seed started to germinate. The ninth day was the key time point for the changes in hormone level during A. rivularis var. floreminore seed germination. In brief, seed germination was not always associated with an increase in GA content or a decrease in ABA content. Abundant sunlight and strong solar radiation in the alpine meadows contribute to the dormancy release during the germination of A. rivularis var. floreminore seeds by, at least partly, affecting hormone levels.

Key words: MODIS BRDF, clumping index, Ross-Li model.