关键词: 生物硝化-反硝化作用, 生物地球化学过程, 富营养化, 生态水文过程, 植物吸收

Abstract: As one of the main drivers of microbial community structure and function, virus is of great significance in studying the ecologically evolutionary processes of microbial community in animal’s intestine. In this study, the ecological characteristics of intestinal phage were explored using Drosophila melanogaster as a model with random PCR amplification of polymorphic DNA (RAPD-PCR) virus fingerprints technique. The results showed that the diversity of phage in wild-type D. melanogaster was higher than that of InR mutant D. melanogaster model. InR mutant D.melanogaster intestinal phage may undergo a short-term disturbance after hatching into adults, with the intestinal phage diversity of day 15 being significantly lower than that on day 3. In contrast, no significant changes were found in healthy wild-type fruit flies. The abundances of phages and bacteria were measured using an epifluorescence microscope. The wild-type D.melanogaster free phages and bacteria on day 3 and day 15 were all at a stable state. On the third day, the abundance of the InR mutant D.melanogaster intestinal bacteria was significantly reduced, and the virusbacteria abundance ratio (VBR) was significantly higher than other groups. On the 15th day, the diversity of InR mutant D.melanogaster phage was reduced, and VBR was also significantly lower than other groups. Therefore, the results of this study indicated that there was a significant difference between the InR mutant D.melanogaster phage and the wild-type phage. Moreover, the random polymorphic DNA polymerase chain reaction (RAPD-PCR) virus fingerprint technology is applicable for the rapid assessment of enterovirus diversity.

Key words: biological nitrification-denitrification, biogeochemical process, eutrophication, eco-hydrological process, plant assimilation.