褪黑素对镉胁迫下蒿柳光合速率下降的缓解效应

doi:10.13292/j.1000-4890.202411.034

生态学杂志 ›› 2024, Vol. 43 ›› Issue (11): 3255-3265.doi: 10.13292/j.1000-4890.202411.034

褪黑素对镉胁迫下蒿柳光合速率下降的缓解效应

殷佳慧^1,2,李霞³,邹竣竹¹,巨关升¹,宁崴²,李奥¹,王元成¹,孙振元^1*

（¹中国林业科学研究院林业研究所，国家林业局林木培育重点实验室，北京 100091； ²吉林农业大学林学与草学学院，吉林省林草遗传育种重点实验室，长春 130118； ³菏泽学院农业与生物工程学院（牡丹学院），山东菏泽 274000）

出版日期:2024-11-10 发布日期:2024-11-06

Alleviation effect of melatonin on degressive photosynthetic rate in Salix viminalis exposed to cadmium.

YIN Jiahui^1,2, LI Xia³, ZOU Junzhu¹, JU Guansheng¹, NING Wei², LI Ao¹, WANG Yuancheng¹, SUN Zhenyuan^1*

(¹Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; ²Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun 130118, China; ³College of Agriculture and Bioengineering (Peony Institute), Heze University, Heze 274000, Shandong, China).

Online:2024-11-10 Published:2024-11-06

摘要/Abstract

摘要： 蒿柳（Salix viminalis）具有较强的镉（Cd）富集能力，但对高浓度Cd耐性低。研究褪黑素（MT）对高浓度Cd条件下蒿柳光合速率的调控作用，有助于揭示MT对木本植物Cd胁迫的缓解效应，为重金属污染植物修复提供栽培保障措施。以无性系蒿柳扦插苗为试验材料，设置空白（BT）、Cd胁迫（CT）、Cd胁迫后叶施MT（MCT）3个处理，明确MT对Cd胁迫下蒿柳气体交换参数、动态光下叶绿素荧光参数、1,5-二磷酸核酮糖羧化酶/加氧酶（Rubisco）含量等的调控模式。结果表明：在处理后第7天，Cd胁迫下蒿柳叶绿素a（Chla）和Rubisco含量分别降低了20%和21%；在处理后第14天，Cd胁迫下蒿柳叶绿素b（Chlb）、气孔导度（G_s）、胞间二氧化碳浓度（C_i）和净光合速率（P_n）分别降低了32%、35%、23%和21%，叶施50 μmol·L^-1 MT后Chla、Chlb、G_s、C_i和Rubisco含量分别提高了30%、28%、47%、35%和25%；在处理后第21天，Cd胁迫下蒿柳P_n和光系统Ⅱ（PSⅡ）最大光化学效率（F_v/F_m）分别降低了36%和16%，叶施MT后P_n和F_v/F_m分别提高了39%和13%；蒿柳受Cd胁迫后光抑制加剧，叶施MT减轻了光抑制。在Cd胁迫下，MT通过提高蒿柳气孔导度促进了蒿柳的光合气体交换，通过维持叶绿素含量促进了其光能吸收，通过提高光能转化效率及光合电子传递速率提升了其光合能力，通过提高Rubisco含量促进了其CO₂同化；MT通过提高光合作用强度促进了Cd胁迫下蒿柳的生长。

关键词: 褪黑素, 镉（Cd）, 蒿柳, 光合速率

Abstract: Salix viminalis has an outstanding capacity to accumulate cadmium (Cd), but with poor tolerance to high concentrations of Cd. Investigating the regulation function of melatonin (MT) on photosynthetic rate of S. viminalis exposed to high concentrations of Cd would help reveal the alleviation effect of MT on woody plants exposed to Cd and provide supporting measures for phytoremediation of heavy metals. Cutting seedlings of clonal S. viminalis were used as experimental material. Three treatments were set as blank (BT), Cd stress (CT), and foliar spraying MT followed by Cd stress (MCT). We examined the regulatory modes of MT on photosynthetic gas exchange parameters, chlorophyll fluorescence parameters under dynamic light, and the content of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in S. viminalis under Cd stress. The chlorophyll a (Chla) and Rubisco contents of S. viminalis exposed to Cd were decreased by 20% and 21% on the 7th day after treatment, respectively. The chlorophyll b (Chlb), stomatal conductance (G_s), intercellular CO₂ concentration (C_i), net photosynthetic rate (P_n) of S. viminalis exposed to Cd were decreased by 32%, 35%, 23%, 21% on the 14th day after treatment. Under the treatment of spraying 50 μmol·L^-1 MT, Chl a, Chlb G_s, C_i, Rubisco content were increased by 30%, 28%, 47%, 35%, 25% respectively. P_n and photosystem Ⅱ (PSⅡ) maximum photochemical efficiency (F_v/F_m) of S. viminalis exposed to Cd stress were decreased by 36% and 16% on the 21st day after treatment. After MT spraying, P_n and F_v/F_m were increased by 39% and 13%, respectively. Photoinhibition of S. viminalis exposed to Cd was aggravated, while spraying MT alleviated its photoinhibition. Under Cd stress, MT promoted photosynthetic gas exchange of S. viminalis by increasing stomatal conductance, promoted light harvesting by maintaining chlorophyll concentration, improved photosynthetic capacity by enhancing light energy conversion efficiency and photosynthetic electron transfer rate, and promoted CO₂ assimilation by increasing Rubisco content. Moreover, MT promoted growth of S. viminalis exposed to Cd stress by increasing photosynthetic intensity.

Key words: melatonin, cadmium (Cd), Salix viminalis, photosynthesis rate

殷佳慧, 李霞, 邹竣竹, 巨关升, 宁崴, 李奥, 王元成, 孙振元. 褪黑素对镉胁迫下蒿柳光合速率下降的缓解效应[J]. 生态学杂志, 2024, 43(11): 3255-3265.

YIN Jiahui, LI Xia, ZOU Junzhu, JU Guansheng, NING Wei, LI Ao, WANG Yuancheng, SUN Zhenyuan. Alleviation effect of melatonin on degressive photosynthetic rate in Salix viminalis exposed to cadmium.[J]. Chinese Journal of Ecology, 2024, 43(11): 3255-3265.

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褪黑素对镉胁迫下蒿柳光合速率下降的缓解效应

Alleviation effect of melatonin on degressive photosynthetic rate in Salix viminalis exposed to cadmium.

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