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生态学杂志 ›› 2021, Vol. 40 ›› Issue (5): 1233-1243.doi: 10.13292/j.1000-4890.202105.015

• 研究报告 • 上一篇    下一篇

种植密度对马尾松人工林土壤磷转化功能微生物与细菌群落结构的影响

潘复静1*,陈英倩1,梁月明2,马姜明3,杨章旗4,颜培栋4,零天旺5,李明金5   

  1. 1桂林理工大学, 环境科学与工程学院, 广西环境污染控制理论与技术重点实验室科教结合科技创新基地, 广西桂林 541000; 2中国地质科学院岩溶地质研究所, 自然资源部、广西壮族自治区岩溶动力学重点实验室, 广西桂林 541000;3珍稀濒危动植物生态与环境保护教育部重点实验室, 广西桂林 541000;4广西壮族自治区林业科学研究院, 南宁 530000; 5广西壮族自治区横县镇龙林场, 南宁 530000
  • 出版日期:2021-05-10 发布日期:2021-05-07

Effects of stand density on community structure of soil phoDharboring microorganisms and bacteria in Pinus massoniana plantations.

 PAN Fu-jing1*, CHEN Ying-qian1, LIANG Yue-ming2, MA Jiang-ming3, YANG Zhang-qi4, YAN Pei-dong4, LING Tian-wang5, LI Ming-jin5   

  1. (1College of Environmental and Engineering, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology for Science and Education Combined with Science and Technology Innovation Base, Guilin University of Technology, Guilin 541000, Guangxi, China; 2Key Laboratory of Karst Dynamics, Ministry of Natural and Resources & Guangxi Zhuangzu Autonomy Region, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541000, Guangxi, China; 3Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin 541000, Guangxi, China; 4Guangxi Forestry Research Institute, Nanning 530000, China; 5Zhenlong Forest Farm of Hengxian County, Nanning 530000, China).
  • Online:2021-05-10 Published:2021-05-07

摘要: 森林植被生长普遍受土壤磷素养分限制。研究不同种植密度人工林土壤磷素组分特征与磷转化功能微生物群落结构的关系,可为选择合适的人工林经营措施提供理论依据。以广西南宁市横县镇龙林场长期定位试验样地的4种密度(低密度(2500株·hm-2)、中密度(3300株·hm-2)、中高密度(4500株·hm-2)和高密度(6000株·hm-2))马尾松林为对象,利用HiSeq2500高通量测序方法分析土壤磷转化功能微生物和细菌的群落结构变化,结合针叶和土壤的生态化学计量学探讨不同种植密度对马尾松林土壤磷转化功能微生物群落结构的影响。结果表明:土壤有机碳和针叶P含量在中密度林较高,而土壤碳氮比值、硝态氮、交换性钙含量、6种土壤磷组分和两种土壤磷酸酶以及针叶C∶P值和N∶P值则相反,说明中密度马尾松林的磷素限制程度比其他种植密度低。土壤磷转化功能微生物和细菌群落结构在不同密度林间存在显著差异,但两者的Shannon多样性指数则不存在显著差异。另外,土壤磷转化功能微生物中占优势的慢生根瘤菌属的相对丰度在中密度林中最小。冗余分析(RDA)发现,土壤磷转化功能微生物和细菌群落结构在不同密度林分之间的显著差异也受到土壤因子的影响,说明土壤磷转化功能微生物和细菌群落组成的差异是林分密度和土壤因子共同作用的结果,但林分密度的影响更大。上述结果表明,中密度马尾松人工林受磷素养分胁迫较小,是一种比较合适的马尾松人工林营林措施。

关键词: 马尾松, 密度, 针叶, 土壤磷组分, 磷转化功能微生物

Abstract: Forest growth is commonly limited by soil phosphorus (P) availability. Understanding the relationships between soil P availability and the community structure of soil phoD harboring microorganisms and bacteria in different stand densities of Pinus massoniana plantations would provide a theoretical basis for selecting an appropriate management strategy. We analyzed the community structures of soil phoDharboring microorganisms and bacteria under four density stands of Pinus massoniana: low density (2500 trees·hm-2), medium density (3300 trees·hm-2), mediumhigh density (4500 trees·hm-2), and high density (6000 trees·hm-2) stands. We used the HiSeq 2500 high-throughput sequencing method to analyze soil microbial community. In addition, the effects of stand density on the community structures of microorganisms were analyzed from an ecological stoichiometry perspective. The results showed that the concentrations of soil organic C and needle leaf P were the highest in the medium-density stands. Soil C∶N ratios, nitrate concentration, exchangeable calcium concentration, six P fractions (CaCl-P, citrate-P, HCl-P, total available P, enzyme-P, and microbial biomass P), acid and alkaline phosphomonoesterase activity, and needle leaf C∶P and N∶P ratios were the lowest in the medium-density stands. The community structure of soil phoDharboring microorganisms and bacteria were significantly different among the four density stands. The Shannon diversity of the microbes was not significantly different among the stands. The relative abundance of the dominant genus Bradyrhizobium was the lowest in the mediumdensity stand. Results of redundancy analysis highlighted the role of soil pH and nutrients in driving variations in the community structures of soil phoDharboring microorganisms and bacteria among different density stands, indicating that the differences in community structures of soil phoDharboring microorganisms and bacteria were driven by stand density and soil factors, with greater contribution from stand density. These results suggest that medium-density Pinus massoniana plantation is least limited by P availability, and thus is a suitable plantation density.

Key words: Pinus massoniana, density, needle leaf, soil P fraction, phoDharboring microorganisms.