• 研究报告 •

### 3种不同类型口器的昆虫取食对菜豆叶片PPO和PAL活性的影响

1. （贵州大学昆虫研究所， 贵州省山地农业病虫害重点实验室， 贵阳 550025）
• 出版日期:2018-11-10 发布日期:2018-11-10

### Effects of feeding of insects with three types of mouthparts on the activities of polyphenols oxidase (PPO) and phenylalnine ammonialyase (PAL) in leaves of kidney bean plant.

YUE Wen-bo, ZHI Jun-rui*, ZHANG Tao, YE Mao, HOU Xiao-lin

1. (Guizhou Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China).
• Online:2018-11-10 Published:2018-11-10

Abstract: To clarify the effects of feedingmodes of different insects on systematic defenses in kidney bean plant, we examined the impacts of damages by tobacco cutworm Spodoptera litura(chewing), spider mite Tetranychus urticae (piercingsucking), and western flower thrips Frankliniella occidentalis (raspingsucking) after 6, 24, 48, and 96 h of feeding middle leaves on activities of polyphenols oxidase (PPO) and phenylalnine ammonialyase (PAL) in the damaged middle leaves and undamaged upper and lower leaves of kidney bean plant. The results showed that PAL and PPO activities were significantly influenced by treatment types, periods of time and leaf position (P<0.01), with significant interactions among the three factors. The activities of PPO in middle leaves increased significantly at 6 h in all different treatments (P<0.05), and changed in different patterns with time extension. The PAL activity in middle leaves was not significantly activated until 48 h after mechanical damage (CK). The activities of PAL in middle leave fed by S. litura, T. urticae and F. occidentalis increased obviously at 6, 6 and 24 h, and reached the maximum, being 2.66, 1.75 and 2.79 times of the control, respectively. The responses of the PAL and PPO in undamaged upper and lower leaves by herbivore feeding were faster than that by mechanical damage. T. urticae feeding caused the fastest changes of PAL activity. The activities of PAL in the upper and lower leaves by T. urticaefeeding reached the maximum at 6 h, which were 2.72 and 5.07 times of the control, respectively. Our results indicated that herbivore feeding could induce the systematic defenses of kidney bean plant, with the effects of temporal and spatial defenses  related to the types of pests.