氮磷添加对中亚热带常绿阔叶林土壤有效氮和pH值的影响

生态学杂志

氮磷添加对中亚热带常绿阔叶林土壤有效氮和pH值的影响

苏渝钦^1,2,刘何铭^1,2,郑泽梅^1,2*,林莉^1,2,李彬彬^1,2,王希华^1,2#br#

（¹ 华东师范大学生态与环境科学学院，上海 200241； ^{2 }浙江天童森林生态系统野外科学观测研究站，浙江宁波 315114）

出版日期:2016-09-10 发布日期:2016-09-10

Effects of N and P addition on soil available nitrogen and pH in a subtropical forest.

SU Yu-qin^1,2, LIU He-ming^1,2, ZHENG Ze-mei^1,2*, LIN Li^1,2, LI Bin-bin^1,2, WANG Xi-hua^1,2#br#

(¹School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China; ^2 Tiantong National Observation Station of Forest Ecosystem, Ningbo 315114, Zhejiang, China).

Online:2016-09-10 Published:2016-09-10

摘要/Abstract

摘要： 为了确定施肥类型和施肥时间对中亚热带常绿阔叶林土壤有效氮和pH值的影响，通过在浙江天童木荷林（Schima superba）设置4种施肥处理（对照：不添加；氮添加：100 kg N·hm^-2·a^-1；磷添加：15 kg P·hm^-2·a^-1和氮磷添加100 kg N·hm^-2·a^-1+15 kg P·hm^-2·a^-1），测定了氮磷添加后森林土壤有效氮和pH值的变化。结果表明：1）氮添加显著促进了土壤铵态氮和硝态氮含量的增加，增加幅度随着施氮时间的延长而增加；磷添加对土壤铵态氮含量无显著影响，但能显著降低土壤硝态氮含量；氮磷添加显著增加土壤硝态氮含量，随着施氮时间的延长，氮磷添加显著增加土壤铵态氮。2）随着施氮时间的延长，氮添加和氮磷添加均能显著降低土壤pH值；磷添加对土壤pH值无显著影响。3）相对硝态氮含量的增加而言，相同程度的土壤铵态氮含量的增加后土壤pH值降低的幅度更大。

关键词: 氮沉降, 细菌群落, Lefse分析, 高通量测序

Abstract: In order to understand the effects of N and P addition on soil available nitrogen and pH in a subtropical evergreen broadleaved forest dominated by Schima superba, we investigated contents of soil NH₄⁺-N, NO₃^--N and pH. Four N and P addition treatments were included as follows: CK (no addition of N and P), N (50 kg N·hm^-2·a^-1), P (15 kg P·hm^-2·a^-1) and NP (100 kg N·hm^-2·a^-1+15 kg P·hm^-2·a^-1). The results showed that: 1) The soil NH₄⁺-N and NO₃^--N concentrations were significantly increased after N addition, and the range of stimulation increased with prolonged nitrogen fertilization time. The soil NO₃^--N concentration was significantly decreased by P addition, and there was no significant impact on soil NH₄⁺-N concentration under P addition. NP addition significantly increased the soil NO₃^--N concentration, and N addition alone had more obvious effects on the soil NO₃^--N concentration than NP addition. The response of soil NH₄⁺-N concentration to NP addition turned from insignificant to significant over time. 2) The soil pH decreased obviously with prolonged N addition and NP addition time, while remained stable under P addition. 3) Relative to the increase of nitrate nitrogen concentration, the same increase of soil ammonium nitrogen concentration resulted in a greater decline in pH value.

Key words: bacterial community, Lefse analysis., high-throughput sequencing, nitrogen deposition

苏渝钦1,2,刘何铭1,2,郑泽梅1,2*,林莉1,2,李彬彬1,2,王希华1,2. 氮磷添加对中亚热带常绿阔叶林土壤有效氮和pH值的影响[J]. 生态学杂志.

SU Yu-qin1,2, LIU He-ming1,2, ZHENG Ze-mei1,2*, LIN Li1,2, LI Bin-bin1,2, WANG Xi-hua1,2. Effects of N and P addition on soil available nitrogen and pH in a subtropical forest.[J]. cje.

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