关键词: 净光合速率, 高通量测序, 群落多样性, 细菌16S rRNA

Abstract: Contingent valuation method (CVM), owing to its flexibility, wide applicability, and mighty data resources, has become one of the most widely used techniques in estimating environmental resource value. However, due to the short time of CVM being introduced to China, it is lack of a set of applicable principles to guide the empirical studies in China, leading to a lot of problems and controversies concerning CVM, such as the validity and reliability of the estimate results. It is believed that CVM is still a promising method to estimate environmental resource value under the premise of extremely careful and proper conduction of the survey, including questionnaire design, survey execution, and data processing. From the three key steps of questionnaire design, implement, and statistic analysis, and from the aspects of core estimate issue, initial bid, totality and sample, survey way, statistic method of willing to pay (WTP), regression model of the factors affecting WTP, and validity and reliability of CVM, this paper analyzed the key techniques of using CVM to improve the estimation of environmental resource value. The possible biases existing in CVM were summarized from the valuation method itself, questionnaire details, implementing process, and data statistics, and the corresponding solutions were proposed.

Key words: high throughput, bacterial 16S rRNA gene, community diversity., net photosynthesis rate