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镉对不同品种苜蓿种子萌发及幼苗生长的影响

保琼莉1,唐一然2,保万魁3*,宋正国1,黄益宗1   

  1. 1农业农村部环境保护科研监测所, 天津 300191; 2天津农学院, 天津 300392;3中国农业科学院农业资源与农业区划研究所, 北京 100081)
  • 发布日期:2020-05-10

Effects of cadmium on seed germination and seedling growth of different alfalfa varieties.

BAO Qiong-li1, TANG Yi-ran2, BAO Wan-kui3*, SONG Zheng-guo1, HUANG Yi-zong1   

  1. (1Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjing 300191, China; 2Tianjin Agricultural University, Tianjing 300392, China; 3Institute of Agricultural Resource and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China).
  • Published:2020-05-10

摘要: 通过培养皿滤纸萌发实验,研究了不同Cd2+浓度(0、5、10、20、50、100、200 mg·L-1)对7种苜蓿种子萌发和幼苗生长的影响。结果表明,5~20 mg·L-1的Cd2+处理对不同品种苜蓿的萌发指标有不同程度的促进效应,10 mg·L-1的处理显著增加了甘农1号和阿尔冈金的鲜重。Cd2+对不同品种苜蓿萌发指标的抑制效应差异较大:Cd2+浓度5~200、20~200、50~200和200 mg·L-1的处理分别显著降低了皇后、牧歌、中兰1号和中兰2号的发芽势;50~200、100~200和200 mg·L-1的处理分别显著降低了中兰1号、牧歌(阿尔冈金)及中兰2号(皇后)的发芽率;50~200、100~200和200 mg·L-1的处理分别显著降低了中兰1号、牧歌(阿尔冈金)和中兰2号(皇后)的发芽指数;20~200 mg·L-1的处理均显著降低了所有品种苜蓿的活力指数;50~200 mg·L-1的处理均显著降低所有品种的鲜重,不同Cd2+浓度对7种苜蓿的根长和/或芽长均有显著抑制效应。所有萌发指标中,发芽率、发芽指数和鲜重与Cd2+浓度之间的Pearson相关性达到极显著水平,Cd浓度的变化对这些指标变化的解释量均超过83%。利用隶属函数值综合评价7种苜蓿对Cd2+的耐受性,皇后以其高的发芽率及Cd2+耐受性,可作为修复重金属污染土壤的优推品种。

关键词: 土壤容重, 土地整治, 土壤呼吸, 微团聚体, 玉米产量

Abstract: The effects of Cd2+ with different concentrations (0, 5, 10, 20, 50, 100, 200 mg·L-1) on seed germination and seedling growth of seven alfalfa varieties were examined in a germination experiment with Petri dish filter paper. The results showed that germination indices of different alfalfa varieties were promoted by different extents with Cd2+ treatments in the range of 5-20 mg·L-1. The fresh weight of Gannong No. 1 and Algonquin was significantly increased under 10 mg·L-1 treatment. The inhibiting effects of Cd2+ treatments on the examined indices of different alfalfa varieties were greatly different. The germination potential of Empress, Amerigraze, Zhonglan No. 1 and Zhonglan No. 2 was significantly decreased under 5-200, 20-200, 50-200 and 200 mg·L-1 treatments, respectively. The germination rates and germination indices of Zhonglan No. 1, Algonquin (Amerigraze), and Zhonglan No. 2 (Empress) were significantly reduced under treatments with Cd2+ concentration of 50-200, 100-200 and 200 mg·L-1, respectively. Cd2+ concentration of 20-200 mg·L-1 significantly decreased vigor index of all varieties. Fresh weight of all varieties significantly were decreased under treatments with Cd2+ concentrations of 50-200 mg·L-1. All Cd2+ treatments significantly inhibited root and/or bud length of seven varieties. The correlations between germination rate, germination index, fresh weight and Cd2+ concentration were significant, with R2 values being higher than 0.83. The tolerances of seven alfalfa varieties to Cd2+ stress, as evaluated comprehensively using the average value of subordinate function of all measurement indices, followed an order of Gannong No. 1 >Empress > Zhonglan No. 2 > Tianshui Alfalfa > Algonquin > Amerigraze > Zhonglan No. 1. In conclusion, the Empress, with high germination rate and tolerance to Cd2+ stress, could be recommended as potential candidate variety for the remediation of cadmium contaminated soil.

Key words: soil respiration, maize yield, soil bulk density, microaggregates., land restoration