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O3和CO2浓度升高对油松针叶抗氧化系统的影响

卢涛1,2;何兴元1;陈玮1   

  1. 1中国科学院沈阳应用生态研究所, 沈阳 110016;2中国科学院研究生院, 北京 100039
  • 收稿日期:2008-12-29 修回日期:1900-01-01 出版日期:2009-07-10 发布日期:2009-07-10

Effects of elevated O3 and CO2 on Pinus tabulaeformis antioxidant system.

LU Tao1,2;HE Xing-yuan1;CHEN Wei1   

  1. 1Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110016, China;2Graduate University of Chinese Academy of Sciences, Beijing 100039, China
  • Received:2008-12-29 Revised:1900-01-01 Online:2009-07-10 Published:2009-07-10

摘要: 利用开顶箱模拟大气O3与CO2浓度升高,对油松进行了连续4个月的熏蒸实验,探讨了油松针叶抗氧化系统化系统的响应。结果表明:1)高浓度O3显著增加了油松针叶过氧化氢的积累,到处理后期过量的过氧化氢显著地抑制了抗氧化酶活性,如SOD和APX,并且抗坏血酸被耗竭,加剧了膜质过氧化,最终导致了严重氧化伤害;2)高浓度CO2处理中油松针叶抗氧化酶活性普遍低于对照,ASA含量显著高于对照,可能是高CO2浓度促进ASA合成,或者是ASA的消耗减少,到处理后期使H2O2含量比对照降低了15.5%,从而减轻了膜质过氧化产物丙二醛含量,减轻了氧化伤害;3)与O3单因素相比,在协同处理中油松针叶具有较高的抗氧化酶活性和ASA含量,说明高CO2浓度减轻了高O3对抗氧化酶活性的抑制作用,并且提高了针叶内ASA含量,增强了针叶的抗氧化能力,有效地控制了ROS的产生与清除平衡,缓解了高O3带来的氧化伤害。

关键词: 两系杂交水稻, 持水量, 叶绿素含量, 产量, 相关关系

Abstract: Pinus tabulaeformis seedlings were planted in opened top chambers (OTCs) and exposed to 80 nmol·mol-1 of O3, 700 μmol·mol-1 of CO2, and 80 nmol·mol-1 of O3 plus 700 μmol·mol-1 of CO2 for four months to study the effects of elevated O3 or/and CO2 on their antioxidant system. Elevated O3 increased the H2O2 content in P. tabulaeforims needles significantly, which in turn depressed the activities of antioxidant enzymes such as SOD and APX, and exhausted the important antioxidant ASA, leading to a serious lipid peroxidation in the late exposure period. Elevated CO2 lowered the antioxidant enzyme activities and enhanced the ASA content in P. tabulaeformis needles, possibly due to its promotion effect on the synthesis of ASA or its reduction effect on ASA consumption, and thus, alleviated the oxidative stress, which was validated by the significantly lower H2O2 content and lipid peroxidation product (MDA) in the needles in the late exposure period. Comparing with those exposed to elevated O3, the P. tabulaeformis needles exposed to elevated O3 plus elevated CO2 had higher antioxidant enzyme activities and ASA content, illustrating that elevated CO2 abated the depression of antioxidant enzyme activities and the exhaustion of ASA content in P. tabulaeformis needles caused by elevated O3, and subsequently, alleviated the O3-induced oxidative damage in P. tabulaeformis.

Key words: Two-line hybrid rice, Water retaining capacity, Chlorophyll content, Yield, Correlation