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三种内生真菌对茅苍术组培苗的生长及主要挥发油成分的影响

张波;戴传超;方芳;陈佳昕   

  1. 南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室, 南京 210046
  • 收稿日期:2008-08-01 修回日期:1900-01-01 出版日期:2009-04-10 发布日期:2009-04-10

Effects of three species endophytic fungi on Atractylodes lancea growth and its essential oil composition.

ZHANG Bo;DAI Chuan-chao;FANG Fang;CHEN Jia-xin   

  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China
  • Received:2008-08-01 Revised:1900-01-01 Online:2009-04-10 Published:2009-04-10

摘要: 采用3种内生真菌与茅苍术组培苗共培养技术,研究了内生真菌对茅苍术组培苗生长及其主要挥发油成分的影响。结果表明:培养4周后,接内生真菌小菌核菌(AL3)的植株平均单株根数最多,比对照多92.7%(P<0.01),并极显著地提高了平均单株根系干质量,比对照高62.9%(P<0.01);而接种内生真菌小克银汉霉(AL4)和孔球孢霉(AL12)对茅苍术组培苗的生长影响不显著;AL3、AL4和AL12极显著提高了茅苍术叶片PAL酶活力,分别比对照提高了5.8、8.0和11.1倍(P<0.01);AL3处理组的POD(过氧化物酶)、CAT(过氧化氢酶)和SOD(超氧化物歧化酶)3种酶活性与对照相似;而AL4处理组的3种酶活性分别比对照降低了9.1%、37.1%和9.7%,AL12处理组的3种酶活性分别比对照提高了10.4%、21.2%和9.6%;AL3、AL4和AL12都对茅苍术叶片总多酚含量有极显著影响,分别比对照提高了179.8%、86.2%和84.1%(P<0.01);接3种内生真菌都改变了挥发油中4种主要活性成分苍术酮、苍术醇、β-桉叶醇及苍术素的相对百分含量。

关键词: 钙, 植物, 土壤肥力, 逆境胁迫, 抗性

Abstract: In a tissue culture experiment, this paper studied the effects of inoculating three species endophytic fungi on the growth and essential oil composition of Atractylodes lancea. After four weeks culture, inoculation of AL3 (Sclerotium sp.) increased the average root number and dry mass per host plant by 927% and 629% (P<001), respectively, while inoculation of AL4 (Cunninghamella sp.) and AL12 (Gilmaniella sp.) had less effect on host plant growth, compared with the control. Inoculating AL3, AL4, and AL12 increased the leaf PAL activity of A. lancea by 58, 80, and 111 times, respectively (P<001). The leaf POD, CAT and SOD activities were at the same levels as the control in treatment AL3, decreased by 91%, 371%, and 97% in treatment AL4, and increased by 104%, 212%, and 96% in treatment AL12, respectively. Inoculating AL3, AL4, and AL12 increased the total polyphenol content of A. lancea by 17977%, 8619%, and 8408%, respectively, and changed the relative percentage of four important components (atractylone, hinesol, β-eudesmol, and atractylodin) in essential oil.

Key words: Calcium, Plant, Soil fertility, Stress, Stress tolerance