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人参与病原菌互作代谢产物醇溶性主成分分析

李勇;丁万隆   

  1. 中国医学科学院北京协和医学院药用植物研究所, 北京 100193
  • 收稿日期:2008-06-05 修回日期:1900-01-01 出版日期:2009-02-10 发布日期:2009-02-10

Principal components analysis of alcohol-soluble metabolites of Panax ginseng interacted with pathogens.

LI Yong;DING Wan-long   

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
  • Received:2008-06-05 Revised:1900-01-01 Online:2009-02-10 Published:2009-02-10

摘要: 采用营养液培养的方法研究了病原菌侵染对生长初期人参根系分泌物收集物组成的影响,分别从对照及菌核菌、疫霉菌、立枯丝核菌、锈腐菌诱导处理的根系分泌物收集物中检测到27、23、17、16和20种主成分,并用标准化合物对其中的11、6、6、3和9种化合物进行了验证。结果表明:病原菌诱导后人参根系分泌物收集物主成分数量明显减少;收集物中化合物类型也发生了明显变化,新增化合物多为有机酸酯、苯及酚酸类衍生物,而减少的化合物无明显规律。

关键词: 分子应答, mRNA差异显示, 水稻, UV-B

Abstract: By the method of nutrient solution cultivation, this paper studied the effects of pathogens infection on the root exudates collections at the early growth stage of Panax ginseng. In the collections of the control and the treatments infected with Sclerotinia schinseng, Phytophthora cactorum, Rhizoctonia solani, and Cylindrocarpon destructans, 27, 23, 17, 16, and 20 principal components were detected, and 11, 6, 6, 3, and 9 of them were validated with standards, respectively. The results showed that under the effects of pathogens infection, the number of principal components in Panax ginseng root exudates collections decreased significantly, and the kinds of the compounds varied evidently. The newly produced compounds were mainly of organic esters, benzoic and phenolic derivatives, while the disappeared compounds were miscellaneous.

Key words: Molecular responses, mRNA differential display, Oryza sativa L., UV-B radiation