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阳春砂仁(Amomum villosum)花气味传粉前后的变化及其生态意义

张亭1,2,杨培1,郭岩伟3,赵荣华3,彭艳琼1*   

  1. (1中国科学院西双版纳热带植物园热带森林生态学重点实验室, 昆明 650223;2中国科学院大学, 北京 100049;3云南中医学院, 昆明 650500)
  • 出版日期:2018-12-10 发布日期:2018-12-10

Variation of floral scent composition of Amomum villosum before and after pollination and its ecological significance.

ZHANG Ting1,2, YANG Pei1, GUO Yan-wei3, ZHAO Rong-hua3, PENG Yan-qiong1*   

  1. (1Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China; 2University of Chinese Academyof Sciences, Beijing 100049, China; 3Yunnan University of Traditional Chinese Medicine, Kunming 650500, China).
  • Online:2018-12-10 Published:2018-12-10

摘要: 在植物有性繁殖中,花气味是吸引传粉昆虫到访,获得传粉、受精的重要化学信号。阳春砂仁(Amomum villosum)是我国四大南药之一,自然结实率低是限制其增产的瓶颈。探索阳春砂仁开花吸引传粉昆虫的化合物是解决结实率低的前提基础。因此,本研究采用动态顶空吸附法分别收集阳春砂仁传粉前后花释放的挥发物,利用气相色谱-质谱(GC-MS)联用技术分析鉴定其成分,对比研究两者间的异同,并分析其生态意义。结果表明:从阳春砂仁花释放的挥发物中共鉴定出22种化合物,主要由萜类、芳香族化合物和脂肪酸衍生物构成。传粉前后阳春砂仁花挥发物显著分化,肉豆蔻酸异丙酯、酞酸二乙酯、壬醛、双环吉马烯、2,3-二氢-4-甲基-呋喃、α-法尼烯和十三烷在昆虫传粉后消失,且γ-榄香烯、D-柠檬烯、β-水芹烯、α-蒎烯和2-十一酮在传粉后含量减少;传粉后新增2-甲基-5-(1-甲基乙基)-2-环己烷-1-酮、3,7-二甲基-1,6-辛二烯-3-醇和4-亚甲基-1-(1-甲基乙基)-双环\[3.1.0\]己烷,并且苯甲酸己酯、β-月桂烯、叔丁基对甲酚和4,7-二甲基十一烷含量增加。这些传粉前后变化的物质可能是引起传粉昆虫行为反应的主要活性化合物,阳春砂仁花通过释放多种化合物,并依靠不同配比实现对传粉昆虫的吸引,其执行的是“泛化”的化学通讯策略。进一步筛选活性化合物,可为改善阳春砂仁传粉效率、增加其产量提供依据。

关键词: 山地, 冠层光合作用, 净生态系统交换, 辐射表, 坡面平行安装, 直角双曲线

Abstract: During the process of sexual reproduction of plants, volatile compounds emitted by flowers play an important role in attracting pollinators who prompt pollination and fertilization of flowers. Amomum villosum is one of the four most famous traditional medicines in south China. The low set rate is the bottleneck of production of A. villosum. Here, the head space dynamic method was used to collect the floral scents from A. villosum before and after pollination. The chemical compositions were analyzed and identified by gas chromatography and mass spectrometry (GC-MS). The results showed that total 22 volatile compounds were found from the floral scents before and after pollination, which were mainly classified into aliphatic, terpenoids and aromatic compounds. The components of volatiles obviously differed before and after pollination. Compared with floral scents before pollination, seven chemical compounds (isopropymyristate, diethyl phthalate, nonanal, bicyclogermacrene, 2,3-dihydro-4-methyl, α-farnesene and tridecane) disappeared, while γ-elemene, β-phellandrene, D-limonene, α-pinene and 2-undecanone compound decreased after pollination. Three chemical compounds \[2-cyclohexen-1-one, 2-methyl-5-(1-methylethenyl), 1,6-octadien-3-ol,3,7-dimethyl, and bicyclo \[3.1.0\] hexane 4-methylene-1-(1-methylethyl)\] newly appeared, and benzoic acid, hexyl ester, β-myrcene, butylated hydroxytoluene and undecane, 4,7dimethyl increased after pollination. These compounds with dramatical changes before and after pollination should be the main active compounds causing the pollinator’s behavioral response. The flowers of A. villosumreleased multiple chemical compounds with different component ratios to attract potential pollinators, which performed the chemical communication of “generalization” strategy. Furthermore, screening active compounds that can effectively attract pollinators may improve the pollination and seed production ofA. villosum.

Key words: canopy photosynthesis, montane, rectangular hyperbola function, net ecosystem exchange, radiometer, slope-parallel installation