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生态学杂志 ›› 2010, Vol. 29 ›› Issue (06): 1208-1212.

• 研究报告 • 上一篇    下一篇

多环芳烃降解菌X20的鉴定及降解特性

杜彦玲1,2;台培东1**;施秋峰3;李培军1   

  1. 1中国科学院沈阳应用生态研究所,沈阳 110016;2中国科学院研究生院,北京 100049;3有色金属华东地质勘查局,南京 210007
  • 出版日期:2010-06-10 发布日期:2010-06-10

Identification and degrading characteristics of PAHs-degrading bacterial strain X20.

DU Yan-ling1,2, TAI Pei-dong1, SHI Qiu-feng3, LI Pei-jun1   

  1. 1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;2Graduate University of Chinese Academy of Sciences, Beijing 100049, China;3Eastern China Geological &|Mining Bureau for Non-Ferrous, Nanjing 210007, China
  • Online:2010-06-10 Published:2010-06-10

摘要: 从多环芳烃降解高效的混合菌群中分离筛选到1株多环芳烃降解菌X20,经形态观察和16S rRNA序列分析,属于假单胞菌(Pseudomonas- sp.)。采用室内摇瓶培养的方法,研究了该菌在不同环境条件下对菲和芘的降解。结果表明:弱碱环境有利于菌株X20对菲和芘的降解,最适pH为8.0;葡萄糖对菲芘降解率的影响呈抛物线变化,当葡萄糖浓度为0.2%时,X20对菲和芘的降解达到最高;X20对菲和芘的降解率随其初始浓度的上升而降低,菲和芘在初始浓度为10、20和40 mg·L-1时的7 d降解率分别为56.3%、39.25%、29.75%和41.8%、29.55%、23.50%,芘对X20降解的抑制强度高于菲。本研究结果将为构建高效的多环芳烃降解菌群,提高多环芳烃原位污染土壤的生物修复效果奠定基础。

关键词: 武夷山风景名胜区, 景观类型, 斑块大小, 概率分布

Abstract: A bacterial strain X20 was isolated from a mixed microflora which can effectively degrade polycyclic aromatic hydrocarbons (PAHs). This strain was identified as Pseudomonas sp., based on its morphological observation and 16S rRNA sequence analysis. The study on the degradation of phenanthrene and pyrene by X20, and the effects of medium pH, PAHs initial concentration, and glucose content on the degradation potential of X20 showed that weak alkaline condition favored the degradation of phenanthrene and pyrene by X20, and the optimal pH was 8.0. The effects of glucose concentrations on the degradation changed as parabolic, and 0.2% was the optimal glucose concentration. When the PAHs initial concentrations were 10, 20, and 40 mg·L-1, the degradation rates of phenanthrene and pyrene were 56.3%, 39.25% and 29.75%, and 41.8%, 29.55% and 23.50%, respectively, i.e., the inhibiting intensity of pyrene to X20 was higher than that of phenanthrene. This study provided a basis for constructing an efficient PAHs-degrading strain and improving the bioremediation effect of PAHs-  in situ contaminated soil.

Key words: Wuyishan scenery district, Landscape type, Patch size, Probability distribution