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CO2浓度倍增对城市油松抗氧化酶活性的影响

阮亚男1,2;何兴元2;陈玮2;陈振举2;孙雨2   

  1. 1辽宁大学生命科学院, 沈阳 110036;2中国科学院沈阳应用生态研究所, 沈阳 110016
  • 收稿日期:2008-10-10 修回日期:1900-01-01 出版日期:2009-05-10 发布日期:2009-05-10

Effects of elevated CO2 concentration on anti-oxidative enzyme activities of urban Pinus tabulaeformis.

RUAN Ya-nan1,2;HE Xing-yuan2;CHEN Wei2;CHEN Zhen-ju2;SUN Yu2   

  1. 1College of Life Sciences, Liaoning University, Shenyang 110036, China;2Institute of Applied Ecology,Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2008-10-10 Revised:1900-01-01 Online:2009-05-10 Published:2009-05-10

摘要: 利用OTC(开顶箱)法模拟未来CO2浓度,于CO2倍增浓度(700 μmol·mol-1)和正常空气CO2浓度(≈350 μmol·mol-1)条件下,测定了沈阳市区油松(Pinus tabulaeformis)针叶超氧自由基(O·2)产生速率、过氧化氢(H2O2)含量、超氧化物歧化酶(SOD)与抗坏血酸-谷胱甘肽循环(ASA-GSH cycle)主要酶活性动态变化,探讨高浓度CO2对油松抗氧化酶活性的影响。结果表明:在短期(60 d)内CO2浓度倍增使油松超氧自由基(O2·)产生速率与过氧化氢(H2O2)含量减少,而SOD、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)活性升高;植株抗氧化能力增强,对活性氧清除能力提高;但长期(70 d以上)CO2浓度倍增处理则可能使试验结果发生逆转。

关键词: 石灰岩, 退化生态系统, 土壤酶

Abstract: Open-topped chamber method was used to study the effects of elevated CO2 concentration (700 μmol mol-1) on the superoxide anion (O〖SX(B-*3〗-〖〗·〖SX)〗2) generation rate, hydrogen peroxide (H2O2) content, and activities of superoxide dismutase (SOD) and crucial enzymes in AsA-GSH cycles in the needles of Pinus tabulaeformis in Shenyang urban area. After a short period (2 months) exposure to the elevated CO2, the superoxide anion generation rate and H2O2 content in P. tabulaeformis needles decreased and the SOD, APX, MDAR, DHAR and GR activities increased, being helpful to the enhancement of P. tabulaeformis anti-oxidative capability. However, a longer period (> 60 days) of exposure might have reverse effects.

Key words: Limestone, Degraded ecosystem, Soil enzyme