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浙闽五个红豆树自然保留种群的遗传多样性

赵颖1;何云芳2;周志春1;冯建国3;金国庆1;王邦顺3   

  1. 1中国林业科学研究院亚热带林业研究所, 浙江富阳 311400;2浙江省林业种苗管理总站, 杭州 310020;3浙江省龙泉市林业科学研究所, 浙江龙泉 323700
  • 收稿日期:2007-11-10 修回日期:1900-01-01 出版日期:2008-08-10 发布日期:2008-08-10

Genetic diversity of five naturally reserved Ormosia hosiei populations in Zhejiang and Fujian provinces.

ZHAOYing1;HE Yun-fang2;ZHOU Zhi-chun1;FENG Jian-guo3, JIN Guoqing1;WANG Bang-shun3   

  1. 1Research Institute of Subtropical Forestry, Chinese Academy of Forestr
    y, Fuyang 311400, Zhejiang, China;2Forest Seed and Seedling Administratio
    n of Zhejiang Province, Hangzhou 310020, China;3Forestry Research Institu
    te of Longquan City, Zhejiang Province, Longquan 323700, Zhejiang, China
  • Received:2007-11-10 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10

摘要: 红豆树是我国三级保护的珍稀濒危植物,是家具、装饰、雕刻等上等用材。应用ISSR分子标记研究浙闽2省5个红豆树自然保留种群的遗传多样性和种群遗传分化。结果表明,红豆树遗传多样性丰富,物种水平的多态位点百分率高达91.46%,总的种群基因多样性为0.3981,显著地高于其他珍稀濒危树种。研究的5个红豆树自然保留种群虽较小,但皆维持较高水平的遗传多样性,种群多态百分率(PPL)、Nei基因多样性(HE)和Shannon信息多样性指数(I)分别为81.71%~89.02%、0.3498~0.3831和0.5026~0.5506。因现有红豆树种群皆是在过渡采伐后保留下来的,片断化时间较短,种群间遗传分化较小,仅有6.45%的遗传变异存在于自然保留种群间,而种群内的变异占总变异的93.55%。较大的红豆树种群遗传多样性相对较高,应优先加强遗传保育。

关键词: 绞股蓝, 蒸腾速率, 气孔导度, 光量子通量密度, 净光合速率

Abstract: Ormosia hosiei is an endangered and precious timber species in South China. Its wood is a top-grade material for furniture, ornament, and sculpture. By using ISSR molecular markers, the genetic diversity and differentiation of five naturally reserved O. hosiei populations in Zhejiang and Fujian provinces were investigated. The results showed thatO. hosiei had a high degree of genetic diversity. The percentage of its polymorphic loci was 91.46%, and the total gene diversity was 0.3981, being significantly higher than those of the other rare and endangered species. Although the five naturally reserved populations were smaller in size, they had a higher level of genetic diversity, with the percentage of population polymorphic loci, Nei’s genetic diversity, and Shannon’s information diversity index being 81.71%-89.02%, 0.3498-0.3831 and 0.5026-0.5506, respectively. The existing populations were all old stands that reserved after excessive deforestation, and the genetic differentiation between these populations was small because of short fragmented time. Molecular variance analysis demonstrated that the among-population and within-population component accounted for 6.45% and 93.55% of the total genetic variation, respectively. Naturally reserved O. hosiei populations with larger size had higher genetic diversity, which would be protected with priority.

Key words: Gynostemma pentaphyllum, Transpiration rate, Stoma conductance, Photon flux density, Net photosynthetic rate