土壤羟胺还原酶活性测定方法的改进

生态学杂志

土壤羟胺还原酶活性测定方法的改进

史云峰^1，2;武志杰¹;史奕¹;陈利军¹;王殳屹^1，2

¹中国科学院沈阳应用生态研究所，沈阳 110016;²中国科学院研究生院，北京 100039

收稿日期:2006-07-11 修回日期:1900-01-01 出版日期:2007-07-10 发布日期:2007-07-10

A modified method for measuring soil hydroxylamine reductase activity.

SHI Yun-feng^1,2; WU Zhi-jie¹; SHI Yi¹; CHEN Li-jun¹; WANG Shu-yi^1,2

¹Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;²Graduate University of Chinese Academy Sciences, Beijing 100039, China

Received:2006-07-11 Revised:1900-01-01 Online:2007-07-10 Published:2007-07-10

摘要/Abstract

摘要： 对传统土壤羟胺还原酶活性的测定方法进行了部分改进。用煮沸并密封冷却的蒸馏水形成3～5 cm的液封，同时用N2气流排除液面以上的空气，能够创造测定土壤中羟胺还原酶活性所需要的厌氧环境。与传统方法相比，该方法减少了厌氧程度的不确定性和试验步骤的复杂性，增加了培养试验的可操作性。以碘量法为对照，对4种不同的测量浸提液中羟胺浓度的分光光度法进行了对比筛选，结果表明,硫酸铁铵-邻菲罗啉法具有较高的准确度和精密度，是测定土壤中羟胺还原酶活性的理想方法。

关键词: 人工林, 苔藓植物生物量, 碳储量, 生态恢复

Abstract: In this paper, the traditional measuring method of soil hydroxylamine reductase activity was partially modified. The anaerobic condition needed for the measurement was created by a 3-5 cm fluid seal, which was made from the boiled water having been cooled down under airproof condition. The air above the fluid seal was exhausted with N₂ current to prevent O₂ dissolving into water. Compared with traditional methods, this method could reduce the uncertainty and the complexity in operation, and increase the maneuverability. Taking iodometric analysis as the reference, four spectrophotometric methods were adopted to determine the hydroxylamine concentration in extract, and the results showed that ammonium ferric sulfate-phenanthroline method was of high accuracy and precision, being feasible in measuring soil hydroxylamine reductase activity.

Key words: Artificial forest, Bryophyte biomass, Carbon storage, Ecological restoration

史云峰;武志杰;史奕;陈利军;王殳屹. 土壤羟胺还原酶活性测定方法的改进[J]. 生态学杂志.

SHI Yun-feng^1,2; WU Zhi-jie¹; SHI Yi¹; CHEN Li-jun¹; WANG Shu-yi^1,2 . A modified method for measuring soil hydroxylamine reductase activity. [J]. cje.

[1]	张国微, 薛建辉, 马洁, 王翰琨. 喀斯特退化山地不同类型人工林土壤养分与酶活性 [J]. 生态学杂志, 2024, 43(3): 616-622.
[2]	杨宬君, 张鑫, 马孟平, 胡一帆, 洪宗文, 熊仕臣, 刘宣, 游成铭. 华西雨屏区不同林龄柳杉人工林表层土壤磷组分特征 [J]. 生态学杂志, 2024, 43(3): 623-632.
[3]	闫明, 刘晓宇, 刘志萍, 刘青青, 奚为民. 特大干旱后美国德克萨斯州东部国家森林碳动态及其驱动因素 [J]. 生态学杂志, 2024, 43(2): 395-406.
[4]	李晓燕, 李永慧, 秦文萍, 孙羽, 马志雯, 刘翔. 人工草地建植对三江源区退化高寒草甸土壤有机碳组分的影响 [J]. 生态学杂志, 2024, 43(1): 50-56.
[5]	徐思若, 成志影, 那雪迎, 张栩嘉, 马大龙, 张鹏. 黑龙江省森林碳汇及其经济价值的变化分析与潜力预测 [J]. 生态学杂志, 2024, 43(1): 197-205.
[6]	胡亚伟, 施政乐, 刘畅, 徐勤涛, 张建军. 晋西黄土区刺槐林密度对林下植物多样性及土壤理化性质的影响 [J]. 生态学杂志, 2023, 42(9): 2072-2080.
[7]	马静, 余克服. 大规模白化对珊瑚礁生态系统的影响研究进展 [J]. 生态学杂志, 2023, 42(9): 2227-2240.
[8]	胡宇科, 赵仲辉, 项文化. 基于涡度相关法的会同杉木林蒸散特征及与彭曼联合法对比 [J]. 生态学杂志, 2023, 42(9): 2266-2275.
[9]	倪添, 谢龙飞, 董利虎. 黑龙江省樟子松人工林含碳量估算方法的比较 [J]. 生态学杂志, 2023, 42(7): 1774-1782.
[10]	郑子潇, 王丹阳, 胡保安, 吴会峰, 韩海荣, 程小琴. 华北落叶松人工林土壤有机碳和团聚体稳定性对间伐的响应 [J]. 生态学杂志, 2023, 42(4): 780-787.
[11]	李俊杰, 韩梦豪, 赵家豪, 胡敬东, 关庆伟. 间伐对马尾松人工林林下植物多样性的动态影响 [J]. 生态学杂志, 2023, 42(4): 788-795.
[12]	杨丽, 李祎, 单博文, 石雷. 竹林土壤碳储量的空间变异分析：以四川长宁县为例 [J]. 生态学杂志, 2023, 42(4): 854-861.
[13]	彭清清, 张耀艺, 张慧玲, 彭艳, 倪祥银, 吴福忠. 中亚热带4种类型森林叶片非生物重金属元素重吸收及累积动态 [J]. 生态学杂志, 2023, 42(12): 2828-2835.
[14]	丁驰, 雷梅, 甘子莹, 王浩, 严强, 丘清燕, 胡亚林. 间伐和施肥对杉木人工林土壤温室气体排放的影响 [J]. 生态学杂志, 2022, 41(6): 1056-1065.
[15]	李姣, 汪杰, 李朗, 周翠烟, 牛潜, 张灿明. 洞庭湖生态经济区土地利用变化对碳储量的影响 [J]. 生态学杂志, 2022, 41(6): 1156-1165.