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重金属污染芒萁居群遗传分化的RAPD分析

李钧敏1;金则新1;朱慧慈2;柯世省1   

  1. 1台州学院生态研究所, 浙江临海 317000;
    2孝顺初中, 浙江金华 321000
  • 收稿日期:2006-03-03 修回日期:2006-06-15 出版日期:2007-02-10 发布日期:2007-02-10

RAPD analysis on the genetic differentiation of Dicranopteris pedata population exposed to heavy metals pollution

LI Jun-min1; JIN Ze-xin1; ZHU Hui-ci2; KE Shi-sheng1   

  1. 1Institute of Ecology, Taizhou University, Linhai 317000, Zhejiang, China;
    2Xiaoshun Middle School, Jinhua 321000, Zhejiang, China
  • Received:2006-03-03 Revised:2006-06-15 Online:2007-02-10 Published:2007-02-10

摘要: 利用随机扩增多态DNA标记技术(random amplified polymorphic DNA, RAPD)对自然生长的芒萁洁净居群和铅锌矿尾矿居群的遗传多样性和遗传分化进行分析。结果表明:在170个检测位点中发现有102个位点呈多态性,在尾矿居群中有2个RAPD标记位点发生了丢失,推测可能为重金属的敏感基因片段;尾矿居群的多态位点百分率(52.94%)、Shannon信息指数(0.3059)和Nei基因多样性(0.2084)均略低于洁净居群(分别为53.53%、0.3196和 0.2198),总的遗传多样性水平较高(分别为70.59%、0.3723和0.2472)。尾矿居群与洁净居群之间发生了一定的遗传分化(Φst=0.1900,Gst=0.1339),但居群间的变异程度远远小于居群内的变异程度,居群间的基因流较大。重金属胁迫对芒萁居群的遗传分化有较大程度的影响,可能是导致芒萁适应重金属污染的分化和微进化的一个重要驱动力。

关键词: 赤潮, 氮, 磷, 比生长速率, 塔玛亚历山大藻

Abstract: By using random amplified polymorphic DNA (RAPD) technique, this paper analyzed the genetic diversity and differentiation of Dicranopteris pedata populations in the habitats with and without mine tailings Pb-Zn pollution. A total of 102 polymorphic loci were detected from 170 loci, and two RAPD-marked loci of the D. pedata population exposed to 20 years mine tailings Pb-Zn pollution were lost, which were presumed to be the gene segments sensitive to heavy metals. The population under mine tailings Pb-Zn pollution had slightly lower percentage of polymorphic loci, Shannon’s information index, and Nei gene diversity than un-polluted one (52.94%, 0.3059 and 0.2084;53.53%, 0.3196 and 0.2198, respectively), but its total genetic diversity was relatively high (70.59%, 0.3723 and 0.2472, respectively). There was a definite genetic differentiation between these two populations (Φst=0.1900, and Gst=0.1339), but the variation degree between the populations was far less than that within the populations, and the gene flow between the populations was high. Pb-Zn pollution had a deeper influence on the genetic differentiation of D. pedata, suggesting that this pollution might be a main driving force in the differentiation and micro-evolution of D. pedata in the process of adapting heavy metals pollution.

Key words: Alexandrium tamarense, Red tide, Nitrogen, Phosphorus, Specific growth rate