流式细胞仪在微型浮游植物生态学中的应用

摘要/Abstract

Abstract: Flow cytometer (FCM) can be used to obtain the muti-parameters of the light scattering and fluorescence of individual cell rapidly and conveniently. It has been more and more applied in oceanographic and limnological studies. The work principles of FCM and its applications in the ecological studies of nano- and pico-phytoplankton were introduced and reviewed based on some recent publications abroad and at home in this paper. By means of FCM, sub-populations of phytoplankton can easily be classified, counted and sorted, and then further studies of the adaptive ability and ecological responses of the sub-populations can be implemented. It was concluded that FCM would have a prosperous prospect in the studies of the primary production and the microbial food web in marine ecosystem.

Key words: planting years of greenhouse, soil microflora, soil enzyme activity.

孙书存, 陆健健, 张利华. 流式细胞仪在微型浮游植物生态学中的应用[J]. 生态学杂志, 2000, (1): 72-78.

Sun Shucun, Jianjian. Applications of Flow Cytometer in Ecological Studies of Nano- and Pico-phytoplankton[J]. cje, 2000, (1): 72-78.

参考文献

[1] 王斌,陆健健.湿地水体浮游植物的流式细胞术研究.见:朗慧卿等编.中国湿地研究与保护上海:华东师范大学出版社.1998.375-381.
[2] 宁修仁,D.沃洛.长江口及其毗连东海水域蓝细菌的分布和细胞特性及其环境调节.海洋学报,1991,13(4):552-559.
[3] 宁修仁,D.沃洛.英吉利海峡蓝细菌增长速率和被摄食速率的测定.海洋学报,1992,14(1):84-93.
[4] 宋平根,李修文主编.流式细胞术的原理和应用.科学出版社.1992.
[5] 张利华.流式细胞仪在浮游植物研究中的应用.东海海洋,1998,16(1):59-62.
[6] Burkill, P.H.et al.Biogeochemical cycling in the northwestern Indian Ocean: a brief overview.Deep - Sea Re search Ⅱ, 1993, 40(3):643-649.
[7] Burkill, P.H.et al.Syneehocoecus and its importance to the microbial foodweb of the northwestern Indian Ocean.Deep- Sea Research Ⅱ, 1993, 40(3):773-782.
[8] Button, D.K.and Roberston, B.R.Kinetics of bacterial processes in natural aquatic systems based on biomass as determined by high- resolution flow cytormetry.Cytometry, 1989, 10:558-563.
[9] Campbell, J.W.and Yentsch, C.M.Variance within homogeneous phytoplankton populations, Ⅲ: analysis of natural populations.Cytometry, 1989, 10:605-611.
[10] Chisholm, S.W.et al.A novel free- living prochlorophyte abundant in the oceanic euphoric zone.Nature, 1988, 334:340-343.
[11] Cho, B.C.and Azam, F.Major role of bacteria in biogeochemical fluxes in the ocean' s interior.Nature, 1988, 332:441-443.
[12] Cucci, T.L.et al.Using phytoplandkton and flow cytometry to analyze grazing by marine organisms.Cytometry, 1989, 10:659-669.
[13] Day, J.W.et al.Estuarine ecology.John Wiley & Sons, Inc.1989.
[14] Del Giorgio, P.A.Et al.Flow cytometry determination of bacterial abundance in lake plankton with the green ncleic acid stain Syto 13.Limnol.O-ceanogr, 1996, 41(4):783-789.
[15] Dorsey, J.etal.Rapid analytical technique for the assessment of cell metabolic activity in the marine microalgae.Cytometry, 1989, 10:622-628.
[16] Dubelaar, G.B.etal.Optical plankton analyzer: a flow cytometry for plankton analysis, Ⅱ: specificaion Cytometry, 1989, 10:529-539.
[17] Ducklow, H.W.Bacterioplankton distribution and production in the northwestem Indian Ocean and gulf of Oman, September 1986.Deep- sea Research Ⅱ, 1993, 40(3):753-771.
[18] Dusenberry, J.A.and Franhel, S.L.Increasing the sensitivity of a FACScan flow cytometer to study oceanic pico - phytoplankton.Limno.Oceanogra., 1994, 39(1):209-559.
[19] Frankel, D.S.etal.Use of a neural net computer system for analysis of flow cytometric data a phytoplankton populations.Cytometry, 1989, 10:540 -550.
[20] Hofstraat, J.W.et al.Phyutoplankton monitoring by flow cytometry.Journal of Plankton Research, 1994, 16(9):1197-1224.
[21] Jochem, F.J.et al.Productivity regime and phytoplankton size structure in the Arabian Sea.Deep - Sea Research Ⅱ, 1993, 40(3):711-735
[22] Kerr, S.R.theory of size of distribution in ecological community.J.Fish.Res.Board.Can., 1974, 31:1859-1862.
[23] Kirchman, D.L.etal.Biomass and production of heterotropic bacterioplankton in oceanic subarctic pacific.Deep - Sea Research I , 1993, 40 (5): 967-988.
[24] Knoechel, R.and Quinn, E.M.Carbon dynamics of logarithmetic and stationary phase phytoplankton as determined by track autoradiography.Cytometry, 1989, 10:612-621.
[25] Krempin, D.W.and Sullivan, C.W.The seasonal abundance, distribution and the relative microbial biomass of chrococcoid cyanobacteria at a station in southern California coastal waters.Can.J.Mircrobial, 1991, 27:1341- 1344.
[26] Le bouteiller, A.et al.Size distribution pattern of phytoplankton in the westeru Pacific: towards a generation for tropical open ocean.Deep- Sea Research, 1992, 39:501-509.
[27] Lesser, M.P.Photobiology of natural populations of Zooxanthellae from the sea Anemone Aiptasia pallida: assessment of host's role in protection against ultraviolet radiation.Cytormetry, 1989, 10: 653-658.
[28] Li, W.K.W.Shipboard analytical flow cytometry of oceanic ultraphytoplankton. Cytometry, 1989, 10:564-579.
[29] Li, W.K.W.Primary production of prochlorophytes, cyanobaceria, and eucaryotic ultraphytoplankton: measures from cytomertric sorting.Limno.Oceanogra., 1994, 39(1):169-175.
[30] Miyazono, A.et al.Seasonal functions of cell density of cyanobacteria and other picophytoplankton in Iwanai bay, Japan.Journal of Oceanography, 1992, 48:257-266.
[31] Murphy, L.S.and Haugen, E.M.the distribution and abundance of phototropic ultraphytoplankton in the north Atlantic.Limno.Oceanogra., 1985, 30:47-58.
[32] Olson, R.J.et al.Pigments, size and distribution of synechococcus in the north Atlantic and Pacific Ocean.Limnol.Oceanogra., 1990, 35:45-58.
[33] Olson, R.J.and Zettler, E.R.Potential of flow cytometry for “pump and probe” fluorescence measurements of phytoplankton photosynthetic characteristics.Limnol.Oceanogra., 1995, 40(4):816 -820.
[34] Partensky, F.et al.Photoacclimation of prochlorococcus sp.(prochlorophyta) strains isolated from the north Atlantic and Mediterranean Sea.Plant Physio., 1993, 101:285-296.
[35] Peeters, J.C.H.et al.Optical plankton analyzer: a flow cytometry for plankton analysis, I: design consideration.Cytometry, 1989, 10:522-588.
[36] Robertson, B.R.and Button, D.K.Characterizing aquatic bacteria according to population, cell size, and apparent DNA content by flow cytometry.Cytometry, 1989, 10:70-76.
[37] S himada, A.et al.Seasonal appearance of prochlorococcus in Suruga bay, Japan in 1992-1993.Journal of Oceanography, 51:289-300.
[38] S ieracki, M.E.et al.Overestimation of heterotrophic bacteria in the Sargraao Sea: direct evidence by flow and imaging cytometry.Deep- Sea Research I, 1995, 42(8):1399-1409.
[39] Trousellier, M.et al.Flow cytometric analysis of coastal lagoon bacterioplankton and picophytoplankton: fixation and storage effects.Estuarine, Coastal and shelf Science, 40:621-633.
[40] Vaulot, D.The importance of prochlorococcus to community structure in the central north Pacific Ocean.Limno.Oceanogra., 1994, 39(4):954-961
[41] Vaulot, D.et al.Simple method to preserve oceanic phytoplankton for flow cytometric analyses.Cytormetry, 198, 10: 629-635.
[42] Vaulot, D.et al.Winter presence of prochlorophytes in surface water of the north western Miditerranean Sea.Limno.Oceanogra., 1990, 35:1156-1164.
[43] Veldhuis M.J.W.and Kraay, G.W.Cell abundance and fluorescence of picophytoplankton in relation to growth irradiance and nitrogen availability in the red sea.Netherlands Journal of Sea Research, 1993, 31(2):135-145.
[44] Waterbury, J.B.et al.Widespread occurrence of a unicellar, marine, planktonic, cyanobacterium.Nature, 1979, 277:273- 294.
[45] Yentsch, C.M.flow cytometry: near real-time information about ocean biology.Oceanography, 1990, 11:47-50.