Abstract: To understand the beneficial fungal resources of sorghum, ITS high-throughput sequencing was used to examine the fungal community structure in different tissues and rhizosphere〖JP〗 soil of mature sorghum in cold region. The results showed that a total of 238 OTUs were obtained, with 88 shared OTUs. Ascomycota and Basidiomycota were the dominant groups of fungi in all the tissues and rhizosphere soil of sorghum. Results of principal coordinate analysis (PCoA) showed that fungi community structure was significantly different among sorghum tissues, while the structure of fungal community in rhizosphere soil was more similar to that in sorghum roots. The richness and diversity of fungal community in rhizosphere soil were significantly higher than those in sorghum tissues. Plant pathogen, wood saprotroph and dung saprotroph were the dominant functional groups in sorghum tissues and rhizosphere soil. Our results provide a basis for further isolation of fungi in sorghum and exploration of the underlying mechanism of fungal community structure in sorghum.

Key words: Sorghum bicolor (L.) Moench, Illumina high-throughput sequencing, fungi, rhizosphere, Internal Transcribed Spacer.