This study was undertaken to evaluate the effects of four different types of artificial seagrass onOryzias melastigmareproduction. The results indicated that daily egg production ofO. melastigma was significantly affected by artificial seagrass. When every two types of seagrass were added together, more eggs were found on globular artificial seagrass or artificial seagrass shaped like sea anemone than on other types of artificial seagrass (P<0.01). When the four types of artificial seagrass were added together, the number of eggs pooled from globular artificial seagrass (176±47 eggs·d^-1) and artificial seagrass shaped like sea anemone (89±32 eggs·d^-1) were significantly higher than that from the others. Besides, the number of eggs presented a reciprocal dynamic trend on different types of seagrass. The number of eggs on artificial seagrass shaped like sea anemone was increased, and eventually exceeded the number of eggs on globular artificial seagrass. It is speculated that marine fish is obviously selective to physical structure of seagrass during the process of laying eggs. Our study provides certain basis for the restoration of fishery resources.