Abstract: To evaluate the diversity of arbuscular mycorrhizal fungi in the rhizosphere of Spiraea pubescens in the Daqingshan Mountain of Inner Mongolia, China, soil samples were collected from three sites Shuimocun, Xiaojinggou and Baishitougou. AMF spores were separated by wetsieving and decanting procedure and centrifugation using sucrose and identified by the morphological methods. The results showed that 48 AMF species were isolated from all soil samples, belonging to 11 genera, and 2 were unidentified species. Glomus was the dominant genus in the three sites. Spore density in soil from Shuimocun was significantly higher than those from Xiaojinggou and Baishitougou. However, the Simpson index and Pielou index in Shuimocun and Xiaojinggou were higher than in Baishitougou. Redundancy analysis of the relationship between soil factors and AMF diversity showed that AMF richness was significantly positively correlated with pH, soil organic carbon and available potassium, and pH was the driving factor influencing AMF richness. Available nitrogen and available phosphorus were significantly negatively correlated with AMF richness and Simpson index.

Key words: soil enzyme activity, micro-irrigation, yield., subsoiling, summer maize