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Isolation and characterization of polychlorinated biphenyl-degrading endophytic bacterium from Salix matsudana f. pendula.

SONG Ge1,2, CAI Man1,2, DU Ke-jiu1,2*   

  1. (1Agricultural University of Hebei, Baoding 071000, Hebei, China; 2Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection, Baoding 071000, Hebei, China).
  • Online:2016-04-10 Published:2016-04-10

Abstract: A total of 12 different endophytic bacterial strains were isolated from Salix matsudana f. pendula in garden and its tissue culture seedlings in this study. Endophytic bacterial strain CGL-1 was identified by using Aroclor 1242 as the only carbon source to grow well. The characteristics of 16S ribosomal DNA sequences and morphology of colony and cell suggested that this isolate was most closely related to the genus Enterobacter. The optimal conditions of Aroclor 1242 degradation were obtained according to the changes of inoculation amount of the bacterium, pH and the concentration of NaCl in liquid minimal media. The inoculation amount of 10% of CGL-1 at pH 7.0 appeared to be the optimum conditions for removing Aroclor 1242. Under these conditions, GCECD was used to measure removal effects of Aroclor 1242 by domesticated strain CGL-1. The results indicated that Aroclor 1242 congeners were obviously removed by strain CGL-1, with a total removal rate of 43.2% after 7 d.

Key words: economic development, Hebei Province, evolution mechanism, ecological environment, coupling degree model