Abstract: The acetone extracts of parsley root materials obtained by four times column chromatography were used for exploring the effects of allelochemicals in the parsley root materials on physiological and biochemical aspects of Fusarium oxysporum f. sp. cucumebrium. Best column fractions were screened by the allelopathic inhibition effect and the activities of three cell wall degrading enzymes and fusaric acid content after 144 h of coculture with F. oxysporum f. sp. cucumebrium in the liquid medium. The results indicated that the best column fractions obtained from the acetone extracts of fresh parsley root and rhizosphere soil inhibited the growth of F. oxysporum f. sp. cucumebrium. In comparison to CK, the cell wall degrading enzymes activities and fusaric acid contents decreased significantly with increased numbers of chromatography. The correlation analysis indicated that allelopathic inhibition effect had significant negative correlations with the activities of pectinase and β-glycosidase enzymes, but no significant correlation with the fusaric acid content.

Key words: altitude, riparian zone, Luanhe Basin, human activity, invasive plant