The effect of soil exogenous ciprofloxacin and copper combined pollution on the early growth of wheat was studied by the toxicological diagnosis method of higher plant. The potential ecological risks of soil contamination derived from livestock manure were investigated. Results indicated the early growth indexes of wheat expressed different degrees of responses to single copper or ciprofloxacin exposure. The wheat shoot and root length were mainly inhibited, while the seed germination rate was unaffected. The sensitivities of the three indexes to the two pollutants were in order of shoot length > root length > germination rate. Ciprofloxacin and copper combined pollution showed different effects on the wheat roots and shoot length. Compared with single copper exposure, inhibition effect on wheat root length was enhanced by the combined treatment of 20 mg·kg^-1 copper and 80 mg·kg^-1 ciprofloxacin, showing a synergistic effect, while the inhibition effect on wheat shoot length by the addition of 200 mg·kg^-1 of copper eased after ciprofloxacin addition, showing an antagonistic effect. The complex, produced by the interaction of ciprofloxacin and copper, may be the cause of various biological effects.