A pot experiment in open top chambers was conducted to investigate the effect of elevated CO₂ on the uptake of Cu and Cd by five Japonica rice varieties (Qianchonglang-2, Shennong-9903, Yongfengjinsui, Fuhe-77, and Yuanfeng-6) in a soil contaminated with two levels of Cu and Cd. The results showed that under elevated CO₂, Cu uptake by the rice varieties except Qianchonglang-2 in the lowly and highly-contaminated soils decreased by 20.6%-30.2% and 2.4%-12.3%, while Cd uptake increased markedly except Yuanfeng6 by 3.7%-297.4% and 31.7%-209.1%, respectively. Although the uptake of Cd by Yuanfeng6 decreased in the highlycontaminated soil, its Cd content still exceeded the China’s national hygienic limit for foods (Cd≤0.2 mg·kg^-1). Additionally, elevated CO₂ increased the values of target hazard quotients (THQ) for Cd toxicity to rice with the THQ >1 in Yongfengjinsui and Yuanfeng-6, which implied that elevated CO₂ would increase the Cd risk of rice to human body health in contaminated soils.