Abstract: Screening relative species SSRs is one of the main methods for obtaining microsatellite makers. Based on the interspecific transferability of Pinus EST-SSRs, this paper screened out the EST-SSRs of Pinus koraiensis from the microsatellite markers of P. massoniana, and analyzed the population genetic diversity with the known Pinus genomic SSRs and EST-SSRs. The results showed that the interspecific transfer rate between P. massoniana and P. koraiensis was 27.27%, and the transferability in Pinus subgenus was lower. As compared with genomic SSRs, the numbers of alleles (N_a), effective alleles (N_e), observed heterozygosity (H_o), and expected heterozygosity (H_e) of EST-SSRs were obviously lower. The genetic diversity analyses of four populations through two sets of markers indicated that the two sets of effective alleles could reflect the differences in the genetic variation trend between the four populations, and gene diversity could also reflect the differences in the genetic identity between the four populations, which demonstrated that the two groups of the markers could accurately reflect the genetic diversity levels of the four populations, and the newly screened EST-SSRs could be used to analyze the population genetic variation.

Key words: soil enzyme activity, naphthalene, soil respiration, soil nutrient, subalpine forest.