Abstract: In order to supply reference for the rational breeding and transportation of Coilia nasus, a laboratory experiment was conducted to measure the oxygen consumption rate (OCR) and asphyxiation point (AP) of juvenile C. nasus under different water temperature, and to study the diurnal variations of the OCR as well as the effects of water temperature on the blood glucose and muscle and liver glycogen of the juveniles. At water temperature (T, ℃) 16-28 ℃, the OCR (R, mg·L^-1) of the juveniles increased with increasing temperature, and there was a linear relationship between the R and T (R=0.988T²-32.36T＋343.7, R²=0.96). The OCR revealed a diurnal rhythm, with the peak at 7:00 am and the bottom at 9:00 pm. The AP (A, mg·L^-1) was significantly correlated to T (A=0.9397T^0.8001, R²=0.98). At water temperature 16-24 ℃, the blood glucose content of the juveniles kept relatively steady and ranged from 17.24 to 19.79 mmol·L^-1,  while the muscle and liver glycogen content fluctuated. At water temperature 24-28 ℃, the blood glucose content increased significantly with increasing temperature, while the muscle and liver glycogen content had a significant decrease.