Abstract: An investigation was conducted on the bryophytes in Zhangjiawanzi gold deposit area of Guizhou Province, and the fluorescence spectroscopy (AF-640) and absorption spectrophotometer AAnalyst 800 were used to measure the concentrations of 6 heavy metals (Cu, Zu, Cd, Pb, Hg, and As) in the dominant bryophyte species and soils in heavily polluted area, relatively polluted area, and clean area. A total of 75 bryophyte samples were collected, belonging to 23 species, 16 genera, and 9 families. The dominant families were Pottiaceae and Bryaceae, whose life-forms were mainly of short turfs, tall turfs, wefts, and mats. The short turfs were significantly dominant, taking up 80.3% of the total life-forms. The measurement of the heavy metals concentrations indicated that this gold deposit area was heavily polluted by Hg, As, and Cd. The enrichment coefficient of Cd by Cymnostomum subrigidulum and Plagiomnium cuspidatum was 5.58 and 1.64, belonging to intensive and relative enrichment, respectively. The concentrations in the bryophytes and soils had a significant positive correlation, suggesting that bryophytes could be used for the monitoring of heavy metals pollution in this gold deposit area.