Application of molecular eco-techniques to treatment system of wastewater

Abstract

Abstract: More attention has been paid on molecular microbial ecology.It is an interdisciplinary field of molecular biology and microbial ecology,which has been used in wastewater treatment.The composition and genetic diversity of microbial communities in wastewater treatment systems were thus reviewed and some special functional microorganisms were described in details.Future trends of molecular ecological techniques combined with wastewater treatment were also discussed.It emphasized on the importance of researching on molecular ecology of microorganisms in treatment system of wastewater so as to understand microbial phylogenetic placement thoroughly.

Key words: Solar greenhouse, Continuously cropped soil, Phenolic acids, Microflora, Enzyme activity

CLC Number:

Q938.1

ZHANG Qianru, ZHOU Qixing, CHEN Xishi, ZHANG Huiwen. Application of molecular eco-techniques to treatment system of wastewater[J]. cje, 2003, (3): 49-53.

References

[1] 周启星,黄国宏.2001.环境生物地球化学及全球环境变化[M].北京:科学出版社.
[2] 孙铁珩,周启星,李培军.2001.污染生态学[M].北京:科学出版社.
[3] 钟鸣,周启星.2002.微生物分子生态学技术及其在环境污染研究中的应用[J].应用生态学报,13(2):247～251.
[4] 须藤隆一(日).1988.水环境净化及废水处理微生物学[M].北京:中国建筑工业出版社.
[5] Amann, Lemmer RH, Wagner M. 1998. Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques[J]. FEMS Microbiol. Ecol., 25: 205～215.
[6] Cangelosi GA, Brabant WH. 1997. Depletion of pre-16S rRNA in starved Escherichia coli cells [J]. J. Bacteriol., 179: 4457～4463.
[7] Cart E, Eason H, Feng S, et al. 2000. RAPD-PCR typing of Acinetobacter isolates from activated sludge systems designed to remove phosphorus microbiologically [J]. J. Appl. Microbiol.,90(3) :309～319.
[8] Cech JS, Hartman P. 1990. Glucose induced break down of enhanced biological phosphate removal[J]. Environ. Technol., 11:651～656.
[9] Cech JS, Hartman P. 1993. Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems[J]. Water. Res., 27:1219～1225.
[10] Daniel B, Oerther, Jakob P, et al. 2000. Monitoring precursor 16SrRNAs of Acinetobacter spp. in activated sludge wastewater treatment systems [J]. Appl. Environ. Microbiol., 66: 2154～2165.
[11] Dionisi HM, Layton AC, Harms G, et al. 2002. Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR [J]. Appl. Environ. Microbiol., 68: 245～253.
[12] Henriksen SD. 1976. Moraxella, Neisseria, Branhamella, and Acinetobacter[J]. Annu. Rev. Microbiol., 30: 63～83.
[13] Holgerm D, Niels BR, Karl HS, et al. 2001. Cultivation-independent, semiautomatic determination of absolute bacterial cell numbers in environmental samples by fluorescence in situ hybridization[J]. Appl. Environ. Microbiol., 67: 5810～5818.
[14] Howarth R, Unz RF, Seviour EM, et al. 1999. Phylogenetic relationships of filamentous sulfur bacteria (Thiothrix spp. and Eikelboom type 021N bacteria) isolated from wastewater-treatment plants and description of Thiothrix eikelboomii sp. nov.,Thiothrix unzii sp. nov., Thiothrix fructosivorans sp. nov. and Thiothrix defluvii sp. nov [J]. Int. J. Syst. Bacteriol., 49:1817～1827.
[15] Juretschko S, Timmermann G, Schmid M, et al. 1998. Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: nitrosococcus mobilis and nitrospira-like bacteria as dominant populations [J]. Appl. Environ. Microbiol., 64:3042～3051.
[16] Kohne DE. 1984. Method for detecting, identifying, and quantitating organisms and viruses[P]. European Patent, :0531798.
[17] Manz W, Wagner M, Amann R, et al. 1994. In situ characterization of the microbial consortia active in two wastewater treatment plants[J]. Water Res., 28:1715～1723.
[18] Maszenan AM, Seviour R J, Patel BKC, et al. 1998. The hunt for the G-bacteria in activated sludge biomass[J]. Water Sci. Technol., 37: 65～69.
[19] Mino T, van Loosdrecht MCM, Heijene JJ. 1998. Microbiology and biochemistry of the enhanced biological phosphorus removal process[J]. Water Res., 32: 3193～3207.
[20] Nielsen PH, de Muro MA, Nielsen JL. 2000. Studies on the in situ physiology of Thiothrix spp. present in activated sludge[J].Environ. Microbiol . , 2(4) :389～398.
[21] Nole SC, Ward DM. 1996. Photosynthate partitioning and fermentation in hot spring microbial mat communities[J]. Appl.Environ. Microbiol., 62: 4598～4607.
[22] Oerther DB, de los Reyes FL, Raskin L. 1999. Interfacing phylogenetic oligonucleotide probe hybridizations with representations of microbial populations and specific growth rates in mathematical models of activated sludge processes[J]. Water Sci. Technol.,39:11～20.
[23] Raskin L, Rittmann B, Stahl DA. 1996. Competition and coexistence of sulfate-reducing and methanogenic populations in anaerobic biofilms[J]. Appl. Environ. Microbiol., 62: 3847～3857.
[24] Selvaratnam S, Barbara AS, Beverly LM, et al. 1995. Application of reverse transcriptase PCR for monitoring expression of the catabolic dmpN gene in a phenol-degrading sequencing batch reactor[J]. Appl . Environ. Microbiol . , 61:3981～3985.
[25] Snaidr J, Amann R, Huber I, et al. 1997. Phylogenetic analysis and in situ identification of bacteria in activated sludge[J]. Appl. Environ. Microbiol., 63: 2884～2896.
[26] Takahiro K, Yoichi K, Shinobu A, et al. 2000. Phylogenetic analysis ofand oligonucleotide probe development for Eikelboom type 021N filamentous bacteria isolated from bulking activated sludge[J]. Appl.Environ. Microbiol., 66: 5043～5052.
[27] Vigdis T, Jostein G, Fride LD. 1990. High diversity in DNA of soil bacteria[J]. Appl. Environ. Microbiol., 56: 782～787.
[28] Wagner M, Amann R, Lemer H, et al. 1993. Probing activated sludge with oligonucleotides specific for proteobacteria:inadequacy of culture-dependent methods for describing microbial community structure[J]. Appl. Environ. Microbiol., 59:1520～1525.
[29] Wagner M, Amann R, Lemmer H, et al. 1995. In situ identification of ammonia-oxidizing bacteria[J]. Syst. Appl. Microbiol.,18: 251～264.
[30] Wagner M, Erhart R, Manz W, et al. 1994. Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge[J]. Appl. Environ. Microbiol., 60: 792～800.
[31] Wiedmann-al-ahmad M, Tichy HV, Schon G. 1994. Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting [J]. Appl.Environ. Microbiol., 60: 4066～4071.