Abstract: By the method of two-dimensional electrophoresis, this paper studied the effects of cold acclimation and de-acclimation on the protein expression of two Spiraea species with different cold resistance and photosynthesis capability. During cold acclimation and de-acclimation, S. bumalda ‘Goldmound’ produced cold acclimation-induced protein, while S. fritschiana did not. Two-dimensional electrophoresis indicated that after cold acclimation, S. fritschiana and S. bumalda ‘Goldmound’ had different protein expression. Through MS identification, there were three kinds of protein conspicuously expressed, two of which were unknown new proteins, their partial amino acid sequences being VSHLAGFSSNNPK and LKADKPTLLSEAK, and DPNDHPNPFTVK, DGNGFFLYLLDPDSSK, and NGDGMFLYLLDGLESK, respectively, and the other was ascorbate peroxidase, an enzyme closely related to photosynthesis. Cold acclimation made the ascorbate peroxidase in the two Spiraea species changed significantly.

Key words: Rice sheath blight, Niche, Niche breadth