Optimization of environmental factors for the growth of Metarhizium anisopliae var. anisopliae strains MA4 and MAlm1.

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Optimization of environmental factors for the growth of Metarhizium anisopliae var. anisopliae strains MA4 and MAlm1.

YANG La-ying¹;GAN Lu²;LIU Li²;WEN Ming-fu²;HUANG Jun-sheng¹

¹Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agr
iculture of Hainan Province, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China; ²Agronomy College, South China University of Tropical Agriculture,
Danzhou 571737, Hainan, China

Received:2007-08-24 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10

Abstract

Abstract: Metarhizium anisopliae var. anisopliae is a kind of broad spectrum entomopathogenic fungi, and thus, to increase its growth rate and sporification is of important significance. In this study, the environmental factors affecting hypha growth and second generation sporulation of two M. anisopliae var. anisopliae strains MA4 and MAlm1, such as culture temperature, initial pH of culture medium, entire volume of liquid, illumination, and microelements, were determined. The results showed that the optimal growth and sporification conditions for MA4 strain were 28 ℃, pH 7, 75 ml/250 ml installment, 24 h light/day with Mn addition; while those for MAlm1 strain were 28 ℃, pH 9, 75 ml/250 ml installment, 0 h light/day, and Cu addition.

Key words: Water circumstances, Spatial patterns, GIS, Loess Plateau

YANG La-ying¹;GAN Lu²;LIU Li²;WEN Ming-fu²;HUANG Jun-sheng¹ . Optimization of environmental factors for the growth of Metarhizium anisopliae var. anisopliae strains MA4 and MAlm1. [J]. cje.

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Optimization of environmental factors for the growth of Metarhizium anisopliae var. anisopliae strains MA4 and MAlm1.

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