Abstract: The genetic diversity of Reaumuria trigyna populations at different habitats was assessed by means of random amplified polymorphic DNA (RAPD) markers. A total of 95 individuals from 5 populations were studied, and 118 loci were detected by 18 random primers, among which, 105 were polymorphic. The mean proportion of polymorphic loci was 88.98%, indicating the high genetic diversity of the R. trigyna populations. The Shannon information index (0.4966), Nei’s gene diversity index (0.3303), and gene differentiation coefficient among populations (G_st=0.1425) suggested that there were more genetic variations within population than among populations, and the genetic differentiation among populations were 14.25%. Cluster analysis showed that there was no direct correlation between genetic distance and geographic distance. The genetic diversity was related to species traits and habitats. Endangered plant was not certainly displayed by lower genetic variation.

Key words: Solar greenhouse, Continuous cropping, Microflora, Enzyme activity, Cucumber