Abstract: Solid phase microextraction(SPME) technique was applied to collect the biogenic volatile organic compounds(BVOCs) emitted from living samples of tomato and geranium.Aset of collecting devices designed by the authors was used in these experiments too.And then,the BVOCs collected were sent to be analyzed by the chromatogram analysis system.The major components in the BVOCs from tomato samples were identified as α-pinene,α- terpinene,phellandrene,sabinene and some unknown chemicals.And α-pinene,camphene,β-pinene,β-myrcene,limonene,caryophyllene and α-humulene were found in the BVOCs from geranium samples.In contrast,the control experiments which employed the conventional aerating absorption system could not tell the certain volatile chemicals of tomato and geranium because of the complex background.These experiments indicated that the technique and devices established in this research were effective to identify the BVOCs emitted from the living aromatic plants directly,with the advantages such as clean background,convenience,good repeatability and solvent free.So this system can be a valuable method to study the volatile second metabolic chemicals in living plants.

Key words: Phenanthrene, Degradative bacterium, Enzyme activity, Bioremediation