Abstract: By the method of selective enrichment culture, a high efficient polycyclic aromatic hydrocarbons (PAHs)-degrading bacterial strain N12 was isolated from a soil collected from Shenfu Irrigation Area, Liaoning Province, and was immobilized as a microbial agent to remedy the high concentration PAHs-polluted soil in a coking plant. Based on the physiological and biochemical analysis and 16S rRNA sequencing, the N12 was identified as Mycobacterium sp., with the isogenous dioxygenase genes nidA and pdoA2 of other Mycobacterium species. After 30 d degradation test, the removal rates of the individuals of PAHs were all >68%, and the total removal rate of PAHs was 80%, suggesting that the N12 could be used for the remediation of high concentration PAHs-polluted soil.

Key words: Urban green-land system, Landscape structure, Spatial pattern, Environmental monitoring, Air pollution