Multiple headspace solid phase microextraction and analysis of volatiles from Arabidopsis green leaf

Abstract

Abstract: Headspace solid phase microextraction (HS-SPME) has been widely used in qualitative characterization of volatile or semi-volatile organic compounds released by plant samples.The quantitative determination of volatile components in headspace analysis was proved to be difficult due to the so-called "matrix effect" and the complicated diffusion and adsorption processes of analytes.In this paper,a direct quantitative method for monitoring volatiles from Arabidopsis thaliana green leaf was presented,based on multiple HS-SPME.The chemical composition of emissions from Arabidopsis was firstly investigated by using SPME coated with DVB/CAR/PDMS fiber.After the extraction parameters were optimized,the total mass of three main green leaf volatiles was calculated by using the peak area of the first single extraction,combined with the calibration of standard solution.The absolute mass of three green leaf volatiles was from 78.6 to 158.4 ng穏^-1.

Key words: Rice, Water deficit stress, Leaf water potential, Photosynthesis, Dry matter partitioning

CLC Number:

Q949

DENG Xiaojun, DU Jiawei. Multiple headspace solid phase microextraction and analysis of volatiles from Arabidopsis green leaf[J]. cje, 2005, (8): 970-974.

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