Abstract: To assess the eco-health status of the Lishui River Basin of Zhangjiajie City, we conducted two comprehensive investigations of macroinvertebrate community in the spring and autumn of 2023. A total of 105 taxa (species/genus) of macroinvertebrates  were detected in the basin, belonging to 4 phyla, 8 classes and 72 families, with an annual average density of 506 ind·m^-2 and an average biomass of 33.86 g·m^-2. Species number, density, and biomass of macroinvertebrates in autumn were higher than spring. A total of 18 biological metrics were screened by methods of distribution range, discriminant ability, and redundancy. The total taxa, Shannon index, and Hilsenhoff biotic index (HBI) were selected to construct the benthic index of biotic integrity (B-IBI) assessment system of the Lishui River Basin of Zhangjiajie City, with a standard value of health (excellent) of 2.00. 73.8% of the monitoring sites were in “fair” or better eco-health status. The eco-health status in autumn was better than that in spring. The overall eco-health status of the basin was “good”, with the average B-IBI value of the basin was 1.55. The B-IBI assessment system contains abundant information based on the data of macroinvertebrates in spring and autumn, which has stronger comparability and practicability,  setting the stage for ongoing long-term monitoring and evaluation of the Lishui River Basin.

Key words: Lishui River Basin, macroinvertebrate, community structure, index of biotic integrity (IBI), health assessment